【摘要】： 為了研究動情周期不同時期對束縛應激的中樞整合的影響,在雌性大鼠動情周期的不同階段給予1小時束縛應激。應用免疫組織化學法觀測雌性大鼠腦不同區(qū)域c-fos蛋白表達的變化,應用放射免疫分析法檢測血中皮質(zhì)酮、雌二醇、孕酮的變化。結果:在1小時束縛應激組與對照組大鼠,均發(fā)現(xiàn)雌二醇在動情前期明顯高于動情期與動情間期水平,而在動情周期的各期內(nèi),雌二醇水平在1小時束縛應激組與對照組無明顯差別。孕酮在各組大鼠動情間期明顯高于動情前期與動情期水平,在動情周期各期內(nèi),應激組與對照組無明顯差別。皮質(zhì)醇水平在對照組大鼠表現(xiàn)為動情前期高于動情期和動情間期,在各應激組大鼠均表現(xiàn)出較對照組明顯增高,但在動情前期增高更明顯。應激組大鼠在皮質(zhì)、海馬、韁核c-fos表達均較對照組增強;在海馬的CA1、CA2、CA3區(qū)與額前皮質(zhì)、犁狀皮質(zhì)、韁核外側部c-fos表達在動情前期低于動情期和動情間期水平。而韁核的內(nèi)側c-fos表達與對照組相比明顯增高,但在動情周期各期沒有差別。實驗結果:動情周期對束縛應激的中樞整合是有影響的,其中涉及到腦內(nèi)的一些核團,包括海馬、皮質(zhì),尤其是以前未提及的韁核,在應激反應中起很大作用。從而對女性的一些情感疾病如抑郁癥、創(chuàng)傷后精神障礙等提供臨床依據(jù)。
[Abstract]:In order to study the effect of estrus cycle on the central integration of restraint stress in different stages of estrus cycle, female rats were given 1 hour restraint stress at different stages of estrus cycle. The changes of c-fos protein expression in different regions of brain of female rats were observed by immunohistochemical method. The changes of corticosterone, estradiol and progesterone in blood were detected by radioimmunoassay. Results: in 1 hour restraint stress group and control group, estradiol was found to be significantly higher in early estrus than in estrus and estrus, but in each stage of estrus cycle. There was no significant difference in estradiol level between the 1 hour restraint stress group and the control group. The level of progesterone in estrous interphase was significantly higher than that in preestrus and estrus, but there was no significant difference between stress group and control group in estrous cycle. The level of cortisol in the control group was higher than that in the estrus phase and estrus interphase in the control group. The level of cortisol in the stress group was significantly higher than that in the control group, but the increase was more obvious in the preestrus phase. The expression of c-fos in cortex, hippocampus and habenula in stress group was higher than that in control group, and c-fos expression in prefrontal cortex, plough cortex and lateral habenular nucleus was lower than that in estrus and estrus interphase in the preestrus of hippocampus and prefrontal cortex, plow cortex and lateral part of habenula. The expression of medial c-fos in habenular nucleus was significantly higher than that in control group, but there was no difference in each stage of estrus cycle. Results: the estrus cycle has an effect on the central integration of restraint stress, which involves some nuclei in the brain, including hippocampus and cortex, especially habenular nucleus, which has not been mentioned before, and plays an important role in stress response. Therefore, some emotional diseases such as depression, post-traumatic disorder and so on provide clinical basis for women.
【學位授予單位】：吉林大學
【學位級別】：碩士
【學位授予年份】：2006
【分類號】：R33

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