發(fā)布時間：2018-10-08 06:18

【摘要】： 通過對BalB/C小鼠接種以不同方式處理的旋毛蟲及不同旋毛蟲抗原,再于不同時間荷以A549肺癌細胞,經(jīng)對試驗小鼠體內(nèi)腫瘤的形態(tài)學指標檢測、小鼠死亡率及其與抑瘤率的分析,觀察旋毛蟲對A549肺癌細胞的抑制作用。此外,在ELISA初步確定A549肺癌細胞抗原與旋毛蟲抗原是否存在免疫交叉反應基礎(chǔ)上,通過SDS-PAGE、Western-blot進一步確定兩者相關(guān)抗原分子量大小,最后利用免疫組化對相關(guān)抗原進行定位。 研究結(jié)果發(fā)現(xiàn)未處理的旋毛蟲抑瘤效果最佳(P=0.005),接種11d后荷瘤組的抑瘤效果好于接種7d前荷瘤組(P=0.006);旋毛蟲ES抗原的抑瘤效果好于旋毛蟲可溶性粗抗原(P=0.001);旋毛蟲與A549肺癌細胞間相關(guān)抗原分子量大小為97.4、68KDa;經(jīng)免疫組化定位分析確定相關(guān)抗原產(chǎn)生的抗體可結(jié)合于A549肺癌細胞的表面。
[Abstract]:BalB/C mice were inoculated with Trichinella spiralis and different antigens of Trichinella spiralis in different ways and then charged with A549 lung cancer cells at different time. To observe the inhibitory effect of Trichinella spiralis on A549 lung cancer cells. In addition, the molecular weight of A549 lung cancer cell antigen and Trichinella spiralis antigen were further determined by SDS-PAGE,Western-blot on the basis of the preliminary determination of A549 lung cancer cell antigen and trichinella antigen by ELISA. The results showed that the antitumor effect of untreated Trichinella spiralis was the best (P0. 005). After 11 d inoculation, the inhibition effect of Trichinella spiralis was better than that of P0. 006 before inoculation, the inhibitory effect of Trichinella spiralis ES antigen was better than that of soluble crude antigen of Trichinella spiralis (P0. 001), and that of Trichinella spiralis and A549 was better than that of P0. 006 before inoculation. The molecular weight of the intercellular associated antigen of lung cancer was 97.446 ~ 68KDa.The antibody produced by the relevant antigen could bind to the surface of A549 lung cancer cell by immunohistochemical localization analysis.
【學位授予單位】：吉林大學
【學位級別】：碩士
【學位授予年份】：2007
【分類號】：R392;R734.2

【引證文獻】

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1 于金玲;劉孝剛;;旋毛蟲感染誘導的抗MCF-7腫瘤細胞作用[J];畜牧與獸醫(yī);2012年01期

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