【摘要】： 第一部分新生大鼠神經(jīng)干細(xì)胞的分離、培養(yǎng) 研究背景及目的神經(jīng)干細(xì)胞(neural stem cells, NSCs)是多分化潛能的細(xì)胞,可分化為神經(jīng)系統(tǒng)的神經(jīng)元、星形膠質(zhì)細(xì)胞和少突膠質(zhì)細(xì)胞,在胚胎及成年哺乳類動(dòng)物中廣泛存在。NSC的分離培養(yǎng)是其基礎(chǔ)和應(yīng)用研究的前提,本部分目的在于獲得新生大鼠NSCs,為深入研究其增殖分化奠定基礎(chǔ)。 方法從新生24h內(nèi)大鼠端腦及中腦部位分離NSC,采用體外無(wú)血清懸浮培養(yǎng),以獲得能在體外長(zhǎng)期生存的NSC;通過(guò)單細(xì)胞克隆實(shí)驗(yàn)檢測(cè)其自我更新能力,免疫細(xì)胞化學(xué)檢測(cè)NSC標(biāo)志物nestin表達(dá)及分化為神經(jīng)元和膠質(zhì)細(xì)胞的能力;用細(xì)胞計(jì)數(shù)法研究其生長(zhǎng)特點(diǎn)并繪制生長(zhǎng)曲線。 結(jié)果獲得的NSC能在體外長(zhǎng)期生存,具有很強(qiáng)的增殖能力、自我更新能力,能分化為神經(jīng)元和膠質(zhì)細(xì)胞,具有多向分化能力,表達(dá)nestin,體外傳代至10代細(xì)胞數(shù)量擴(kuò)增了40倍。 結(jié)論成功地從新生大鼠端腦和中腦中分離得到了能在體外長(zhǎng)期
[Abstract]:The first part: isolation and culture of neural stem cells from neonatal rats background and objective Neural stem cells (neural stem cells, NSCs) are multi-differentiated cells. The isolation and culture of neurons, astrocytes and oligodendrocytes, which can differentiate into nervous system, are the premise of the basic and applied research on the separation and culture of .NSC in embryos and adult mammals. The aim of this part is to obtain NSCs, from neonatal rats and lay a foundation for further study of its proliferation and differentiation. Methods NSC, was isolated from rat telencephalon and midbrain within 24 hours and cultured in vitro with serum-free suspension. NSC;, which could survive for a long time in vitro, was tested for its self-renewal ability by single cell clone assay. Immunocytochemistry was used to detect the expression of NSC marker nestin and its ability to differentiate into neurons and glial cells. Results the obtained NSC could survive for a long time in vitro, had strong ability of proliferation, self-renewal, differentiation into neurons and glial cells, and had the ability of multi-directional differentiation. The number of cells subcultured to the 10th passage of the expressed nestin, in vitro was expanded by 40 times. Conclusion\
【學(xué)位授予單位】：重慶醫(yī)科大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2006
【分類號(hào)】：R329.28

【相似文獻(xiàn)】

相關(guān)期刊論文 前10條

1 焦倩;章海霞;呂海俠;劉勇;;新生大鼠器官型腦組織片培養(yǎng)和誘導(dǎo)神經(jīng)干細(xì)胞分化的觀察[J];南方醫(yī)科大學(xué)學(xué)報(bào);2011年08期

2 佟雷;佟曉杰;;JNK信號(hào)轉(zhuǎn)導(dǎo)通路對(duì)神經(jīng)干細(xì)胞增殖的影響[J];解剖學(xué)研究;2011年02期

3 朱登納;賈延R，

本文編號(hào)：2241276