【摘要】：Slit為中樞神經(jīng)系統(tǒng)中線膠質(zhì)細胞所分泌的一類可擴散的大分子蛋白質(zhì)，具有排斥發(fā)育中的神經(jīng)生長錐，誘導細胞遷移，促進軸突生長與分支形成的作用。Robo屬于細胞粘附分子(cell adhesionmolecules，CAMs)免疫球蛋白(immunoglobulin，Ig)超家族成員，為跨膜受體蛋白，介導Slit對生長錐和神經(jīng)元的排斥作用，參與調(diào)控發(fā)育中的中樞神經(jīng)軸突定向，同時Robo也具有同親和附著與異親和附著特性，可促進軸突生長和調(diào)控神經(jīng)纖維束化、定位。有關Slit及Robo在成年動物周圍神經(jīng)系統(tǒng)中的分布與功能報道極少。同時，周圍神經(jīng)再生特異性(再生軸突生長定向)的細胞與分子機制尚不明確。 目的：研究Slit、Robo基因和蛋白質(zhì)在成年大鼠脊髓、脊神經(jīng)節(jié)(dorsal root ganglian，DRG)、坐骨神經(jīng)(sciatic nerve，SN)的表達差異及損傷后的變化，探討它們在神經(jīng)損傷再生過程中的作用，揭示Slit、Robo的生理功能和周圍神經(jīng)再生特異的分子機制。 材料與方法：SD大鼠坐骨神經(jīng)切斷或脊神經(jīng)后根切斷后，存活3、7、14、21、28 d，，取其相應節(jié)段脊髓、后根、DRG和損傷神經(jīng)的遠、近端；利用RT-PCR、原位雜交、免疫組織化學等方法檢測Slit1、Slit2、Robo1、Robo2在上述組織中的表達。進而利用坐骨神經(jīng)壓榨(sciatic nerve crush，SNC)和切斷后立即縫合(end to end suturetransected sciatic nerve，SNS)模型，將可溶性Robo1-Fc融合蛋白注入到損傷或縫合部位，形成坐骨神經(jīng)壓榨實驗組(SNCE)和坐骨神
[Abstract]:Slit is a kind of diffusible macromolecular protein secreted by glial cells in the central nervous system. Promoting axon growth and branching. Robo belongs to the superfamily of cell adhesion molecule (cell adhesionmolecules,CAMs) immunoglobulin (immunoglobulin,Ig), which is a transmembrane receptor protein that mediates the rejection of growth cone and neurons by Slit. It is involved in the regulation of the orientation of central nervous axons during development, and Robo also has the characteristics of homocompatibility and hetero-affinity, which can promote the growth of axons and regulate the bundles of nerve fibers. The distribution and function of Slit and Robo in the peripheral nervous system of adult animals are rarely reported. At the same time, the cellular and molecular mechanisms of peripheral nerve regeneration specificity (regenerative axon growth orientation) are unclear. Objective: to study the expression of Slit,Robo gene and protein in spinal cord, spinal ganglia (dorsal root ganglian,DRG) and sciatic nerve (sciatic nerve,SN) of adult rats and their role in the regeneration of nerve injury. To reveal the physiological function of Slit,Robo and the specific molecular mechanism of peripheral nerve regeneration. Materials and methods after sciatic nerve transection or spinal nerve posterior root transection, the rats survived for 28 days, and the corresponding segments of spinal cord, posterior root and the distal and proximal end of injured nerve were taken from the corresponding segments of spinal cord, posterior root and injured nerve, and RT-PCR, in situ hybridization was used. Immunohistochemical methods were used to detect the expression of Slit1,Slit2,Robo1,Robo2 in these tissues. Then the model of sciatic nerve compression (sciatic nerve crush,SNC) and (end to end suturetransected sciatic nerve,SNS (suture immediately after transection) was used to inject soluble Robo1-Fc fusion protein into the injured or sutured site to form the sciatic nerve compression experimental group (SNCE) and ischium spirit.
【學位授予單位】：中南大學
【學位級別】：博士
【學位授予年份】：2006
【分類號】：R322

【引證文獻】

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