【摘要】：目的觀察復(fù)方鹿角霜對(duì)小鼠膠原誘導(dǎo)性關(guān)節(jié)炎(CIA)體內(nèi)相關(guān)炎癥細(xì)胞因子的表達(dá)及滑膜組織病理學(xué)的影響。方法建立小鼠CIA模型,實(shí)驗(yàn)小鼠隨機(jī)分為5組:空白對(duì)照組,模型對(duì)照組,復(fù)方鹿角霜總膏低、高劑量組(C1,C2組),雷公藤多苷片組。小鼠第一次免疫后,觀察小鼠的一般情況,記錄小鼠前后足爪病變發(fā)生情況。小鼠第二次免疫后,復(fù)方鹿角霜C1、C2組分別灌胃給予復(fù)方鹿角霜總膏2.5,5 g·kg-1·d-1,雷公藤多苷片組灌胃給予雷公藤多苷片13.6 g·kg-1·d-1,連續(xù)4周。小鼠給藥結(jié)束后,取血分離血清,酶聯(lián)免疫吸附試驗(yàn)(ELISA)檢測(cè)小鼠血清腫瘤壞死因子-α(TNF-α)和白細(xì)胞介素-4(IL-4)的表達(dá)水平。取小鼠足踝關(guān)節(jié),光鏡觀察關(guān)節(jié)滑膜組織病理學(xué)變化。結(jié)果與模型對(duì)照組比較,復(fù)方鹿角霜C1、C2組、雷公藤多苷片組血清TNF-α均明顯降低;而復(fù)方鹿角霜組血清IL-4水平升高。模型對(duì)照組關(guān)節(jié)病理可見滑膜組織增生,各給藥組關(guān)節(jié)病理形態(tài)有所改善。結(jié)論復(fù)方鹿角霜可減輕膠原誘發(fā)的小鼠關(guān)節(jié)炎紅腫癥狀,其作用機(jī)制可能與降低炎性因子TNF-α、調(diào)節(jié)抑炎因子IL-4作用有關(guān)。
[Abstract]:Objective to observe the effect of compound Lujiao cream on the expression of inflammatory cytokines and histopathology of synovium in (CIA) of mice with collagen-induced arthritis. Methods CIA model was established in mice. The experimental mice were randomly divided into 5 groups: blank control group, model control group, compound Lujiao cream low, high dose group (C1C 2 group), Tripterygium wilfordii polyglycoside group. After the first immunization, the general situation of mice was observed and the occurrence of paw lesions before and after immunization was recorded. After the second immunization, the compound antler cream C _ 1C _ 2 group was given the compound antler cream 2.5g kg-1 d ~ (-1) by gavage, and the tripterygium wilfordii polyglycoside tablet 13.6 g kg-1 d ~ (-1) by gavage for 4 weeks. Serum samples were collected from mice after administration, and the expression of tumor necrosis factor- 偽 (TNF- 偽) and interleukin-4 (IL-4) in serum of mice were detected by Elisa (ELISA). The serum levels of tumor necrosis factor 偽 (TNF- 偽) and interleukin-4 (IL-4) in mice were determined by enzyme-linked immunosorbent assay. The histopathological changes of synovial membrane were observed under light microscope. Results compared with the model control group, the serum TNF- 偽 of the C _ 1C _ 2 group and the Tripterygium wilfordii polyglycoside group were significantly decreased, while the serum IL-4 level of the compound antler cream group was higher than that of the control group. Synovial tissue proliferation was observed in the model control group, and the joint morphology was improved in each administration group. Conclusion compound Lujiao cream can alleviate the symptoms of arthritis redness and swelling induced by collagen in mice, and its mechanism may be related to the reduction of inflammatory factor TNF- 偽 and the regulation of anti-inflammatory factor IL-4.
【作者單位】： 三峽大學(xué)第一臨床醫(yī)學(xué)院;湖北省宜昌市中心人民醫(yī)院藥劑科;華中科技大學(xué)同濟(jì)醫(yī)學(xué)院藥學(xué)院;湖北中醫(yī)藥大學(xué);
【基金】：國(guó)家“重大新藥創(chuàng)制”科技重大專項(xiàng)項(xiàng)目(2011ZX09102-006-05)
【分類號(hào)】：R285.5;R-332

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