本文選題：精子 + 基因表達�。� 參考：《第一軍醫(yī)大學》2005年博士論文

【摘要】：不孕不育是全世界關注的課題。目前每100對已婚夫婦中,就有10對夫婦存在不孕不育問題。其中男性因素占到了50%。也就是說,男性精子質量不佳,已影響到人類的繁衍生息。 引起精子質量不佳的原因很多,長期以來人們進行了多方面的研究,但病因和發(fā)病機制都不是十分明確,臨床治療效果也不盡如人意。男性精子質量不佳,從而引發(fā)的男性不育,成為困擾臨床的難題之一。從分子水平探明影響精子質量的因素,以便采取有針對性的診斷、治療措施,提高精子質量,提高人類生育力,具有重要的理論和現實意義。 遺傳學的發(fā)展提示我們“人類的疾病或多或少與基因有關”,男性精子質量不佳也應是如此。因此,我們試圖從基因的水平來解釋男性精子質量不佳的原因,以期能夠對臨床采取有針對性的、更有效的診斷、治療措施,提供理論上的支持和指導。從基因的水平闡述疾病的致病機制,可以有兩個途徑。一是從基因組DNA的完整性、正確性入手,研究精子基因組DNA是否存在DNA堿基序列的大片段缺失,或出現DNA堿基的某些位點處的突變、缺失等。另一方面則是著眼于基因的表達,因為人類有3×10~(10)個堿基對,但僅僅表達3萬個左右的蛋
[Abstract]:Infertility is a worldwide concern. Currently, for every 100 married couples, 10 couples have infertility problems. Male factors account for 50% of the total. In other words, male sperm quality is poor, has affected human reproduction. There are many reasons for the poor quality of sperm. For a long time, people have carried out many studies, but the etiology and pathogenesis are not very clear, and the effect of clinical treatment is not satisfactory. The poor quality of male sperm, which leads to male infertility, has become one of the clinical problems. It is of great theoretical and practical significance to identify the factors affecting sperm quality at molecular level in order to take targeted diagnosis and treatment measures to improve sperm quality and improve human fertility. The development of genetics suggests that "human diseases are more or less genetically related", as should the poor quality of male sperm. Therefore, we try to explain the reasons for the poor quality of male spermatozoa from the gene level, in order to provide theoretical support and guidance for clinical diagnosis and treatment. There are two ways to explain the pathogenesis of disease at the gene level. The first is to study whether there are large deletion of DNA base sequence, mutation and deletion at some sites of DNA base in sperm genomic DNA from the integrity and correctness of genomic DNA. On the other hand, it's about gene expression, because humans have 3 脳 10 ~ (10) base pairs, but only about 30,000 eggs.
【學位授予單位】：第一軍醫(yī)大學
【學位級別】：博士
【學位授予年份】：2005
【分類號】：R346

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本文編號：2095266