本文選題：銅綠假單胞菌 + 群體感應(yīng)�。� 參考：《西北大學(xué)》2007年碩士論文

【摘要】： 銅綠假單胞菌(Pseudomonas aeruginosa，PA)是一種機(jī)會(huì)致病菌，它可以在人群中引起嚴(yán)重的急性和慢性感染，是病人在醫(yī)院期間發(fā)生感染的第三大致病菌。許多抗生素對(duì)其具有一定的治療作用，但銅綠假單胞菌的一個(gè)重要特性是它對(duì)許多抗生素具有很高的內(nèi)在抗藥性，因此開(kāi)發(fā)新的抗生素是控制銅綠假單胞菌感染的有效途徑。 群體感應(yīng)調(diào)節(jié)系統(tǒng)(quorum sensing，QS)，是近年發(fā)現(xiàn)的細(xì)菌細(xì)胞之間通過(guò)自誘導(dǎo)物(Autoinducer,AI)信號(hào)分子，來(lái)協(xié)調(diào)群體行為的一種機(jī)制。QS系統(tǒng)廣泛存在于革蘭氏陰性菌和革蘭氏陽(yáng)性菌中，并控制著包括生物被膜形成、毒素產(chǎn)生、致病因子表達(dá)、胞外多糖產(chǎn)生等一系列的細(xì)菌行為和表型，對(duì)病原菌致病過(guò)程起決定性的作用。因此，通過(guò)控制QS系統(tǒng)來(lái)控制病原菌致病是一個(gè)非常有希望的策略。 本研究利用銅綠假單胞菌受群體感應(yīng)系統(tǒng)(QS)調(diào)節(jié)的基因lasI和rhlA為報(bào)道基因，以熒光發(fā)光桿菌(Photorhabdus luminescens)的熒光素酶基因操縱子luxCDABE和蔗糖致死基因sacB為報(bào)道子，構(gòu)建了兩種高靈敏度、低背景、高通量的QSI篩選系統(tǒng)(體系Ⅰ和體系Ⅱ)。利用體系Ⅰ，對(duì)一個(gè)小型中藥成份庫(kù)進(jìn)行了篩選，發(fā)現(xiàn)黃酮類(lèi)成分雙藿苷A對(duì)銅綠假單胞菌las QS系統(tǒng)，有明顯抑制作用。該物質(zhì)可能成為抗致病菌藥物的先導(dǎo)化合物。此篩選體系，，為從中藥及其他天然成分中研究開(kāi)發(fā)新型抗致病藥物，提供了一個(gè)可行的技術(shù)平臺(tái)。
[Abstract]:Pseudomonas aeruginosa PA (Pseudomonas aeruginosa PA) is a opportunistic pathogen, which can cause severe acute and chronic infection in the population. Many antibiotics have a certain therapeutic effect, but one of the important characteristics of Pseudomonas aeruginosa is that it has high intrinsic resistance to many antibiotics. Therefore, the development of new antibiotics is an effective way to control the infection of Pseudomonas aeruginosa. The quorum sensing system (QS) is a mechanism by which the signal molecules of autoinducer AI (Autoinducer AI) are found among bacterial cells to coordinate colony behavior. QS system widely exists in Gram-negative bacteria and Gram-positive bacteria. It also controls a series of bacterial behaviors and phenotypes, including biofilm formation, toxin production, expression of pathogenic factors and production of extracellular polysaccharides, which play a decisive role in the pathogenic process of pathogenic bacteria. Therefore, it is a promising strategy to control pathogenic bacteria by controlling QS system. In this study, two highly sensitive genes of Pseudomonas aeruginosa, Lasi and rhlA, were constructed using luciferase gene operon luxCDABE and sucrose lethal gene SACB of Photorhabdus luminescens as reporter genes. Low background, high throughput QSI screening system (system I and system II). Using system 鈪

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