本文選題：德爾卑沙門菌 + 脈沖凝膠電泳��； 參考：《中國抗生素雜志》2014年10期

【摘要】：目的分析人和豬來源的德爾卑沙門菌的分子同源性,為研究多重耐藥性在人畜之間傳播提供分子生物學(xué)技術(shù)依據(jù)。方法藥敏試驗檢測人和豬來源的德爾卑沙門菌多重耐藥株對10種抗菌藥物的敏感性;用脈沖凝膠電泳方法(PFGE)檢測菌株間的分子同源性;結(jié)合實驗或電轉(zhuǎn)移試驗獲得目標(biāo)質(zhì)粒,質(zhì)粒酶切圖譜檢測質(zhì)粒的同源性。PCR分析染色體介導(dǎo)喹諾酮耐藥基因gyr A和par C,質(zhì)�？山閷�(dǎo)的喹諾酮耐藥基因qnr A/qnr B/qnr C/qnr S、aac(6′)-Ⅰb-cr、qep A和oqx AB。結(jié)果共收集48株德爾卑沙門菌,經(jīng)藥敏試驗篩選獲得11株多重耐藥株(MDR),人源性8株,豬源性3株。11株菌除對氨芐青霉素、氯霉素、鏈霉素、磺胺和四環(huán)素(R型:ACSSu T)耐藥外,所有菌株也對萘啶酸和環(huán)丙沙星耐藥,6株對左氧氟沙星耐藥,5株對頭孢曲松耐藥。PFGE分析,11株菌共分為6個克隆,3株動物源性菌株與8株人源性菌株無克隆相關(guān)性。喹諾酮耐藥基因分析顯示,有5株和3株動物源株檢測到gyr A的Ser83Leu或/和Asp87Asn突變,6株人源株和3株動物源株檢測到par C的Ser80Ile或/和Thr57Ser突變。質(zhì)粒電泳分析顯示,所有菌株都存在1-4條質(zhì)粒條帶,大小介于2~180kb,其中3株人源性菌株和1株動物源性株都存在4kb左右大小的質(zhì)粒;2株動物源性菌株僅存在20 kb大小質(zhì)粒。用結(jié)合實驗和電轉(zhuǎn)移方法,分析4kb質(zhì)粒結(jié)構(gòu),證實4個質(zhì)粒的酶切圖譜完全一致,質(zhì)粒中均存在介導(dǎo)外排基因oqx AB和喹諾酮耐藥基因aac(6′)-Ⅰb-cr。結(jié)論人和豬來源的德爾卑沙門菌多重耐藥株中質(zhì)粒介導(dǎo)的外排基因oqx AB和喹諾酮耐藥基因aac(6′)-Ⅰb-c是喹諾酮重要的耐藥機(jī)制,可能在沙門菌間傳遞,導(dǎo)致對喹諾酮類高耐藥性。通過人和豬的接觸或間接途徑,沙門菌有傳播喹諾酮耐藥性的風(fēng)險,同一種質(zhì)粒可在人和動物中轉(zhuǎn)移而傳播該耐藥機(jī)制。
[Abstract]:Objective to analyze the molecular homology of Salmonella delpeiensis from human and pig, and to provide molecular biological basis for studying the transmission of multidrug resistance between human and animal. Methods the susceptibility of human and porcine multidrug resistant strains to 10 antimicrobial agents was detected by drug sensitivity test, molecular homology was detected by pulsed gel electrophoresis (PGE) method, and target plasmids were obtained by combination of experiments or electrical transfer tests. The homology of plasmids was detected by restriction endonuclease mapping. PCR was used to analyze the chromosome-mediated quinolone-resistant genes gyr A and par C, and the plasmid mediated quinolone-resistant genes qnr A/qnr Br / Qnr C / Qnr Sapaacti-鈪，

本文編號：2046531