本文選題：成纖維細胞樣基質(zhì)細胞 + 不成熟樹突狀細胞　； 參考：《浙江大學(xué)》2006年碩士論文

【摘要】：樹突狀細胞(dendritic cell,DC)是機體內(nèi)功能最強大的抗原提呈細胞(Antigen-presenting cells,APC),是免疫反應(yīng)的啟動者,因為它具有獨特的刺激初始T細胞活化的能力而備受關(guān)注。但是隨著對DC研究的深入,DC細胞群體的異質(zhì)性越來越受到關(guān)注,DC不但可以啟動免疫反應(yīng)而且也能負向調(diào)控免疫反應(yīng)。體內(nèi)不但存在啟動免疫反應(yīng)的DC,同時也存在抑制免疫反應(yīng)的DC。目前對調(diào)節(jié)性DC(Regulatory DC)的研究很多都是在體外進行的,如在原先的DC培養(yǎng)條件下加入各種免疫抑制性因子進而將造血前體細胞、成熟或不成熟DC誘導(dǎo)成具有負向調(diào)控作用的調(diào)節(jié)性DC,但這種方法過于簡化,不能反映體內(nèi)的真實情況,因而其誘導(dǎo)產(chǎn)生的DC可能就不一定代表體內(nèi)的真實細胞亞群。 機體是一個復(fù)雜的系統(tǒng),各個組分在各個層次都可能發(fā)生相互作用,因此,免疫系統(tǒng)肯定也在各個層面受到其他系統(tǒng)的影響而更好發(fā)揮作用。我們以前的研究工作表明脾臟微環(huán)境無論對成熟DC或造血前體細胞都有重要的影響,脾臟基質(zhì)細胞不但可以誘導(dǎo)造血前體細胞分化發(fā)育成為調(diào)節(jié)性DC,還可以誘導(dǎo)成熟DC進一步增殖分化成調(diào)節(jié)性DC。脾臟作為二級淋巴器官是免疫系統(tǒng)的一個組成部分,其微環(huán)境對免疫細胞的影響已經(jīng)得到了一些闡明,那么其他實質(zhì)器官如肺臟、肝臟、腦會不會也對免疫細胞具有同樣的影響呢? 肺臟是機體最大的開放器官之一,每天肺臟都要接觸大量的外界抗原,因此免疫耐受的形成對肺臟尤為重要�；|(zhì)細胞(Stromal cells)作為器官的重要組分,除了其維持器官結(jié)構(gòu)的功能外,它對免疫系統(tǒng)會有什么影響嗎?有報道指出肺臟成纖維細胞與T細胞有比較緊密的聯(lián)系,如成纖維細胞通過PGE2抑制T細胞的活化后死亡(activation induced cell death),那么肺臟基質(zhì)細胞會對DC有什么影響嗎?體外長期培養(yǎng)的細胞系可能有別于體內(nèi)基質(zhì)細胞組分,但仍然可以部分地模擬體內(nèi)的微環(huán)境,而且細胞系使用方便,因此鑒于以上的思考,我們建立了小鼠的肺臟成纖維細胞樣基質(zhì)細胞系,通過該細胞系與DC的共培養(yǎng)體系來研究共培養(yǎng)后的DC的各種變化。
[Abstract]:Dendritic cell (DCC) is the most powerful antigen-presenting cell (APCP) in the body. It is the promoter of immune response and has attracted much attention because of its unique ability to stimulate the activation of initial T cells. However, with the further study of DC, the heterogeneity of DC cell population has attracted more and more attention. DC can not only initiate the immune response, but also negatively regulate the immune response. There are not only DCs that initiate immune response, but also DCs that inhibit immune response. At present, many studies on regulatory DCM are carried out in vitro, such as adding various immunosuppressive factors in the original DC culture condition, which will result in hematopoietic progenitor cells. Mature or immature DCs are induced into regulatory DCs with negative regulatory effects, but this method is too simplified to reflect the real situation in the body. So the DC that it induces may not necessarily represent a real subgroup of cells in the body. The body is a complex system in which the components may interact at all levels, so, The immune system must also be better affected by other systems at all levels. Our previous work has shown that spleen microenvironment has important effects on both mature DC and hematopoietic progenitor cells. Splenic stromal cells can not only induce the differentiation and development of hematopoietic precursor cells into regulatory DCs, but also induce the further proliferation and differentiation of mature DCs into regulatory DCs. The spleen, as a secondary lymphoid organ, is an integral part of the immune system. The effect of its microenvironment on immune cells has been elucidated. Does the brain have the same effect on immune cells? lung is one of the largest open organs of the body. Every day, the lungs are exposed to a large number of external antigens, so the formation of immune tolerance is particularly important to the lungs. Stromal cells (Stromal cells) as an important component of the organ, in addition to its function to maintain the organ structure, how does it affect the immune system? It has been reported that lung fibroblasts are closely related to T cells. If fibroblasts inhibit the activation of T cells through PGE2, then how do lung stromal cells affect DC? Cell lines that have been cultured for a long time in vitro may be different from the components of stromal cells in vivo, but they can still partly mimic the microenvironment in vivo, and the cell lines are easy to use. Therefore, in view of the above considerations, A mouse lung fibroblast-like stromal cell line was established. The co-culture system of the cell line and DC was used to study the changes of DC after co-culture.
【學(xué)位授予單位】：浙江大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2006
【分類號】：R392

【引證文獻】

相關(guān)碩士學(xué)位論文 前2條

1 王文文;肺臟基質(zhì)細胞分泌的VEGF對樹突狀細胞分化發(fā)育的影響[D];泰山醫(yī)學(xué)院;2011年

2 祁雪萍;小鼠骨髓來源調(diào)節(jié)性樹突狀細胞的體外培養(yǎng)和鑒定[D];山西醫(yī)科大學(xué);2012年

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本文編號：2002826