本文選題：女性生殖道 + 子宮內膜��； 參考：《四川大學》2005年碩士論文

【摘要】：[背景] 抗菌肽是天然免疫的重要介質,由機體自身基因編碼產生,具有抗細菌、抗真菌、抗病毒等生物學活性。女性生殖系統也是天然免疫的重要場所,迄今在女性生殖道中已鑒定出有多種抗菌肽或抗菌蛋白分子存在,例如防御素、溶菌酶、乳鐵蛋白、白細胞蛋白酶抑制劑等。是否還存在新的抗菌蛋白或肽分子,目前仍然受到學者們的關注。現今已鑒定出的女性生殖道抗菌肽或抗菌蛋白來源于上皮細胞和/或白細胞,這些天然抗菌分子是否有新的細胞來源,也是令人感興趣的問題。因此本研究對人子宮內膜粘液和子宮頸粘液的酸溶性提取物進行分離純化,力圖鑒定出新的抗菌肽分子,并對所鑒定出的抗菌肽分子的細胞來源、組織學分布也進行了分析。此外,抗菌肽分子具有固有或誘導表達的特點,因此我們對新鑒定出的分子在臨床炎癥和非炎癥狀態(tài)下的表達情況進行了分析,以期進一步了解女性生殖道的天然免疫機制,并為其將來用于臨床女性生殖道感染防治打下基礎。 [方法與結果] 對收集的子宮內膜粘液和子宮頸粘液酸溶性提取物分別進行制備性酸性尿素聚丙烯酰胺凝膠電泳(AU-PAGE),并用電泳瓊
[Abstract]:Background: antimicrobial peptides are important mediators of innate immunity. They are encoded by organism's own genes and have biological activities such as antibacterial, antifungal, antiviral and so on. The female reproductive system is also an important place for innate immunity. Up to now, a variety of antimicrobial peptides or antimicrobial proteins have been identified in the female genital tract, such as defensins, lysozyme, lactoferrin, leukocyte protease inhibitors and so on. Whether there are new antimicrobial proteins or peptide molecules is still concerned by scholars. It is also interesting whether these natural antimicrobial molecules are derived from epithelial cells and / or white blood cells. In this study, acid soluble extracts from human endometrial mucus and cervical mucus were isolated and purified in order to identify new antimicrobial peptides, and the cellular origin and histological distribution of identified antimicrobial peptides were also analyzed. In addition, antimicrobial peptide molecules have inherent or inducible expression characteristics, so we have analyzed the expression of newly identified molecules in clinical inflammatory and non-inflammatory states in order to further understand the innate immune mechanism of female genital tract. And lay a foundation for the prevention and treatment of female genital tract infection in the future. [methods and results] Acid urea polyacrylamide gel electrophoresis (AU-PAGEG) was used to prepare acid urea polyacrylamide gel electrophoresis (AU-PAGEN) from the collected endometrial mucus and cervix myxic acid soluble extracts.
【學位授予單位】：四川大學
【學位級別】：碩士
【學位授予年份】：2005
【分類號】：R392

【引證文獻】

相關期刊論文 前1條

1 張東玲;關瑞章;黃文樹;熊靜;;血紅蛋白源抗菌片段的研究進展[J];水生生物學報;2013年05期

相關碩士學位論文 前1條

1 米寶明;奶牛子宮內膜抗菌多肽的分離與純化[D];甘肅農業(yè)大學;2009年

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本文編號：1936131