發(fā)布時(shí)間：2018-05-08 00:30

  本文選題：ABCG2 + 氣管干細(xì)胞�。� 參考：《中國(guó)醫(yī)科大學(xué)》2006年博士論文

【摘要】：前言 干細(xì)胞是指具有無(wú)限或較長(zhǎng)期的自我更新能力,并能產(chǎn)生至少一種高度分化子代細(xì)胞的細(xì)胞。按照組織發(fā)生學(xué)來(lái)源,可將干細(xì)胞分為胚胎干細(xì)胞和成體干細(xì)胞。近年來(lái)對(duì)成體干細(xì)胞的研究越來(lái)越受到重視,目前已發(fā)現(xiàn)的成體干細(xì)胞有造血干細(xì)胞、骨髓間充質(zhì)干細(xì)胞、神經(jīng)干細(xì)胞、肌干細(xì)胞、角膜干細(xì)胞、胰腺干細(xì)胞、皮膚干細(xì)胞、肝臟干細(xì)胞等,并已不同程度地用于臨床治療。但迄今為止國(guó)際上對(duì)氣管干細(xì)胞的研究還很不完善。先前本研究組已成功地在體內(nèi)外建立了氟脲嘧啶介導(dǎo)的大鼠氣管損傷修復(fù)模型,并對(duì)氣管干細(xì)胞進(jìn)行了初步的定位和性狀解析。 干細(xì)胞具有排出熒光染料Hoechst33342的特性。近年來(lái)的研究表明,干細(xì)胞的這一特性(即SP表型)與ATP結(jié)合盒(ABC)轉(zhuǎn)運(yùn)蛋白超家族成員之一的ABCG2(又名乳癌耐藥蛋白1,Bcrp1)的表達(dá)有關(guān)�，F(xiàn)已證明在多個(gè)物種的多種組織來(lái)源的SP細(xì)胞中檢測(cè)到ABCG2的高表達(dá),如骨髓、肝、肌組織、乳腺上皮及ES細(xì)胞。ABCG2與SP表型的密切關(guān)系,使得ABCG2成為多潛能干細(xì)胞的共同標(biāo)志,對(duì)干細(xì)胞的鑒定具有重要意義。為更深入地了解氣管干細(xì)胞的特性,本研究在5-FU介導(dǎo)的氣管上皮損傷修復(fù)模型的基礎(chǔ)上,應(yīng)用間接免疫熒光法和Wetern blot動(dòng)態(tài)觀測(cè)了修復(fù)過(guò)程中各時(shí)間點(diǎn)氣管上皮ABCG2蛋白的表達(dá),借以原位觀察了氣管干細(xì)胞在整個(gè)修復(fù)過(guò)程中的動(dòng)態(tài)分布。 近年來(lái)的研究表明Wnt信號(hào)及其成員對(duì)胚胎及成體的原始細(xì)胞的增殖和分化具有重要的調(diào)節(jié)作用。為探討氣管干細(xì)胞的增殖分化與Wnt/β-catenin信號(hào)途徑的關(guān)系,闡明干細(xì)胞增殖分化的分子機(jī)制,本研究應(yīng)用RT-PCR及間接免疫熒光的方法動(dòng)態(tài)觀測(cè)了修復(fù)過(guò)程中Wnt信號(hào)相關(guān)成分Wnt1、β-catenin、cyclinD1 mRNA的變化以及β-catenin蛋白的細(xì)胞內(nèi)分布,初步探討了Wnt/β-catenin信號(hào)途徑參與調(diào)控氣管干細(xì)胞增殖
[Abstract]:Preface Stem cells are cells that have unlimited or long-term self-renewal and produce at least one highly differentiated progeny. Stem cells can be divided into embryonic stem cells and adult stem cells according to the origin of histogenesis. In recent years, more and more attention has been paid to the research of adult stem cells. At present, the adult stem cells have been found to be hematopoietic stem cells, bone marrow mesenchymal stem cells, neural stem cells, muscle stem cells, corneal stem cells, pancreatic stem cells, skin stem cells. Liver stem cells and so on, and have been used in clinical treatment to varying degrees. However, the international research on tracheal stem cells is not perfect. The model of rat trachea injury induced by fluorouracil in vitro and in vivo has been successfully established, and the primary localization and character analysis of tracheal stem cells have been carried out. Stem cells are characterized by the emission of fluorescent dye Hoechst33342. Recent studies have shown that this characteristic of stem cells (SP phenotype) is related to the expression of ABCG2 (also known as Bcrp1), a member of the ATP binding cassette transporter superfamily. It has been proved that high expression of ABCG2 has been detected in SP cells from many species, such as bone marrow, liver, muscle, mammary epithelium and es cells. ABCG2 is closely related to SP phenotype, which makes ABCG2 a common marker of multipotential stem cells. It is of great significance for the identification of stem cells. In order to better understand the characteristics of tracheal stem cells, the expression of ABCG2 protein in tracheal epithelium at different time points was dynamically observed by indirect immunofluorescence and Wetern blot on the basis of 5-FU mediated repair model of tracheal epithelial injury. The dynamic distribution of tracheal stem cells during the whole repair process was observed in situ. Recent studies have shown that Wnt signaling and its members play an important role in regulating the proliferation and differentiation of embryonic and adult primordial cells. To investigate the relationship between proliferation and differentiation of tracheal stem cells and Wnt/ 尾 -catenin signaling pathway, and to elucidate the molecular mechanism of stem cell proliferation and differentiation. In this study, RT-PCR and indirect immunofluorescence were used to dynamically observe the changes of Wnt signal related components Wnt1, 尾 -catenin D1 mRNA and the intracellular distribution of 尾 -catenin protein, and to explore the role of Wnt/ 尾 -catenin signaling pathway in regulating the proliferation of tracheal stem cells.
【學(xué)位授予單位】：中國(guó)醫(yī)科大學(xué)
【學(xué)位級(jí)別】：博士
【學(xué)位授予年份】：2006
【分類(lèi)號(hào)】：R329

【參考文獻(xiàn)】

相關(guān)期刊論文 前4條

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本文編號(hào)：1859161