本文選題：血瘀證 + 急性心肌梗死��； 參考：《吉林大學(xué)》2007年碩士論文

【摘要】： 目前的中藥藥理研究,絕大多數(shù)動物實(shí)驗(yàn)還是采用西醫(yī)病因病理復(fù)制動物模型,只強(qiáng)調(diào)“病”,而忽略了“證”,很難通過動物模型反映中醫(yī)“辨證施治”的治療原則和中藥的治療特點(diǎn)。如何將中醫(yī)征候與疾病有機(jī)結(jié)合起來,建立“病”和“證”相統(tǒng)一的實(shí)驗(yàn)動物模型,使之具有可重復(fù)性和操作性,是中藥藥理研究的一個關(guān)鍵技術(shù)平臺。以往在研究治療胸痹心痛(冠心病心絞痛)的中藥時,通常選用西醫(yī)的大鼠急性心肌梗死病理模型進(jìn)行評價,忽視了中醫(yī)的征候。而臨床上的胸痹心痛可見多種中醫(yī)“證侯”,如“血瘀證”。為確證單純病理模型能否準(zhǔn)確反映出不同的中醫(yī)“證侯”,我們建立了大鼠“血瘀證”下急性心肌梗死病理模型,通過NB-T染色計算梗死范圍、心肌三酶、心電變化、自由基及抗氧化酶、內(nèi)皮損傷相關(guān)物質(zhì)、心肌代謝物質(zhì)、“血瘀證”特征因子、血小板粘附性及聚集性、體外血栓形成、紅細(xì)胞壓積及血沉及血流動力學(xué)等多種指標(biāo)與單純心肌梗死的病理模型進(jìn)行比較,同時采用中藥和西藥進(jìn)行藥物干預(yù)。結(jié)果表明,盡管“血瘀證”下急性心肌梗死模型在心肌形態(tài)學(xué)、血液生化學(xué)、血液流變學(xué)、血流動力學(xué)等各方面比“單純”急性心肌梗死模型的變化程度增大,但兩者之間無明顯差異,而且與西藥和中藥的干預(yù)作用結(jié)果相一致。提示大鼠單純心肌梗死模型可以用來評價血瘀證下心肌梗死,無須建立“急性心肌梗死”和“血瘀證”并存的實(shí)驗(yàn)動物模型。但單純西醫(yī)的病理模型是否可以代替中醫(yī)“證”與“病”并存的征候模型,尚無前人報道。
[Abstract]:In the current pharmacological study of traditional Chinese medicine, the vast majority of animal experiments still use Western medicine to make animal models of etiology and pathology, emphasizing only "disease" and neglecting "syndrome".It is difficult to reflect the treatment principle of TCM and the characteristics of TCM by animal model.How to combine TCM symptoms with diseases organically, and establish a unified experimental animal model of "disease" and "syndrome", so as to make it repeatable and operable, is a key technical platform for the pharmacological research of traditional Chinese medicine (TCM).In the past, the traditional Chinese medicine for the treatment of chest arthralgia and angina pectoris was usually evaluated by the pathological model of acute myocardial infarction in rats with western medicine, and the symptoms of TCM were ignored.Clinical chest pain can be seen in a variety of traditional Chinese medicine, such as "blood stasis syndrome."In order to confirm whether the simple pathological model can accurately reflect different TCM "syndromes", we established a pathological model of acute myocardial infarction under "blood stasis syndrome" in rats. The infarct size, myocardial trienzyme and ECG changes were calculated by NB-T staining.Free radical and antioxidase, endothelium-related substances, myocardial metabolites, characteristic factors of "blood stasis syndrome", platelet adhesion and aggregation, thrombus formation in vitro,The hematocrit, erythrocyte sedimentation rate and hemodynamics were compared with the pathological model of myocardial infarction.The results showed that the changes of myocardial morphology, blood biochemistry, hemorheology and hemodynamics in acute myocardial infarction model under "blood stasis syndrome" were larger than those in "pure" acute myocardial infarction model.But there was no significant difference between the two, and the results were consistent with the intervention of western medicine and traditional Chinese medicine.It is suggested that the simple myocardial infarction model can be used to evaluate myocardial infarction under blood stasis syndrome, and it is not necessary to establish an experimental animal model of "acute myocardial infarction" and "blood stasis syndrome".But whether the pathological model of western medicine can replace the symptom model of "syndrome" and "disease" in traditional Chinese medicine has not been reported before.
【學(xué)位授予單位】：吉林大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2007
【分類號】：R-332

【引證文獻(xiàn)】

相關(guān)期刊論文 前1條

1 李勇華;鄭景輝;王麗萍;簡維雄;黃獻(xiàn)平;袁肇凱;;養(yǎng)心通脈有效部位方動員心肌梗塞大鼠MSCs歸巢及對心功能和病理的影響[J];時珍國醫(yī)國藥;2010年09期

相關(guān)博士學(xué)位論文 前3條

1 李勇華;急性心肌梗死心血瘀阻證BMSCs動員歸巢的機(jī)理及養(yǎng)心通脈有效部位方干預(yù)的研究[D];湖南中醫(yī)藥大學(xué);2010年

2 譚琦;加味丹參飲誘導(dǎo)骨髓間充質(zhì)干細(xì)胞分化為心肌樣細(xì)胞的研究[D];湖南中醫(yī)藥大學(xué);2011年

3 王祺;獨(dú)參湯聯(lián)合骨髓間充質(zhì)干細(xì)胞治療急性心肌梗死大鼠的相關(guān)研究[D];遼寧中醫(yī)藥大學(xué);2011年

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本文編號：1766925