本文選題：靜息軟骨細胞　切入點：刀豆蛋白A　出處：《吉林大學》2006年碩士論文

【摘要】：變形性關節(jié)炎等退形性關節(jié)疾病和關節(jié)機械性損傷的發(fā)病率在逐年提高。由于軟骨組織結構的特殊性,缺乏血管,淋巴,神經系統(tǒng)的營養(yǎng)供給,給得軟骨疾病的治療恢復帶來了很大的困難。應用組織工程學技術體外構建人工軟骨,嘗試關節(jié)軟骨損傷的修復是國際上該領域的研究熱點,軟骨細胞也是最早應用于組織工程的種子細胞之一,但由于軟骨細胞作為種子細胞,其來源困難,增殖能力和數量有限,體外長期培養(yǎng)的軟骨細胞易發(fā)生反分化現象,失去軟骨細胞的生物活性,限制了其應用。因此對軟骨的增殖分化機制的研究,尋找強效的刺激因子必然對軟骨組織工程的發(fā)展,軟骨疾病的防治帶來很大的幫助。 刀豆球蛋白(Concanavalin A,簡稱ConA)是Sumner 1919年首次從大刀豆中分離純化出來的一種植物凝集素,后來Sumner和Howell[19]發(fā)現純化的ConA不僅能凝集紅細胞,而且還能沉淀溶液中的糖元和淀粉。隨著ConA的研究的深入,ConA的生物活性也逐漸得到認識,ConA對淋巴細胞具有強烈的促有絲分裂作用,刺激
[Abstract]:The incidence of degenerative joint diseases and mechanical joint injuries such as deformable arthritis is increasing year by year. It is very difficult to treat and recover the cartilage diseases. It is a hot spot in the world to construct artificial cartilage in vitro and try to repair the articular cartilage injury by using tissue engineering technology. Chondrocytes are also one of the first seed cells used in tissue engineering. However, because of the difficulty of chondrocytes as seed cells, their proliferation ability and quantity are limited, the long-term cultured chondrocytes are prone to dedifferentiation. The loss of the biological activity of chondrocytes limits its application. Therefore, the study on the mechanism of cartilage proliferation and differentiation and the search for strong stimulating factors are bound to be of great help to the development of cartilage tissue engineering and the prevention and treatment of cartilage diseases. Concanavalin A (Cona) is the first plant agglutinin isolated from concanavalin by Sumner in 1919. Later, Sumner and Howell [19] found that the purified ConA not only agglutinated red blood cells, Along with the further study of ConA, the biological activity of Cona has been gradually recognized that Cona has a strong mitogenic effect on lymphocytes and stimulates it.
【學位授予單位】：吉林大學
【學位級別】：碩士
【學位授予年份】：2006
【分類號】：R341;R684

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