本文選題：骨髓基質(zhì)干細(xì)胞　切入點(diǎn)：細(xì)胞分化　出處：《廣西醫(yī)科大學(xué)》2006年碩士論文　論文類(lèi)型：學(xué)位論文

【摘要】： 目的:探討體外分離及擴(kuò)增純化兔骨髓基質(zhì)干細(xì)胞的實(shí)驗(yàn)方法,并研究其生物學(xué)特性;以不同誘導(dǎo)液在體外對(duì)BMSCs誘導(dǎo)分化為雪旺樣細(xì)胞,探討以BMSCs作為周?chē)窠?jīng)組織工程種子細(xì)胞的可行性。 方法:采用Percoll(密度為1.073g/ml)分離液和貼壁篩選法,從兔股骨中分離BMSCs進(jìn)行純化、培養(yǎng);原代細(xì)胞按1: 2比例進(jìn)行傳代培養(yǎng);在DMEM-LG + 10%FBS中培養(yǎng),用β-ME、b-FGF和黃芪等為誘導(dǎo)劑對(duì)第1, 3, 5代骨髓基質(zhì)干細(xì)胞進(jìn)行定向誘導(dǎo)分化,倒置像差顯微鏡觀察細(xì)胞形態(tài)學(xué)化,免疫細(xì)胞化學(xué)鑒定S-100蛋白和GFAP的表達(dá)率。 結(jié)果:原代及傳代培養(yǎng)顯示,兔BMSCs具有活躍的增殖能力,第2, 3, 5代細(xì)胞的生長(zhǎng)曲基本相同;誘導(dǎo)后的兔骨髓基質(zhì)干細(xì)胞具有類(lèi)似雪旺細(xì)胞的形態(tài),免疫細(xì)胞化學(xué)鑒定顯示S-100蛋白和GFAP的表達(dá)率(76±6.7)%、(25±5.45)%。 結(jié)論:應(yīng)用本實(shí)驗(yàn)方法培養(yǎng)兔髓基質(zhì)干細(xì)胞,體外生長(zhǎng)穩(wěn)定、增殖速度快、可連續(xù)傳代;采用β-ME、b-FGF和黃芪等為誘導(dǎo)劑,可使兔骨髓基質(zhì)干細(xì)胞在體外向雪旺樣細(xì)胞分化。
[Abstract]:Objective: to investigate the methods of isolating and purifying rabbit bone marrow stromal cells in vitro and their biological characteristics, and to differentiate Schwann like cells into Schwann like cells induced by different inducers in vitro. To explore the feasibility of using BMSCs as seed cells for peripheral nerve tissue engineering. Methods: BMSCs isolated from rabbit femur was purified and cultured by Percollus (density 1.073 g / ml) and adherent screening method, primary cells were subcultured in 1: 2 ratio, and cultured in DMEM-LG 10s. Bone marrow stromal stem cells (BMSCs) of the first, third and fifth passages were induced to differentiate by 尾 -ME-b-FGF and astragalus membranaceus. The morphologic changes of bone marrow stromal cells were observed by inverted aberration microscope. The expression rates of S-100 protein and GFAP were identified by immunocytochemistry. Results: primary culture and passage culture showed that rabbit BMSCs had active proliferative ability, and the growth curve of the 2nd, 3rd and 5th passage cells was basically the same, and the induced rabbit bone marrow stromal stem cells had the morphology similar to Schwann cells, and the growth curve of rabbit bone marrow stromal cells was similar to that of Schwann cells. The expression rate of S-100 protein and GFAP was 76 鹵6.7% and 25 鹵5.45% by immunocytochemistry. Conclusion: rabbit marrow stromal stem cells can be cultured in vitro with stable growth, rapid proliferation and continuous passage, and 尾 -ME-b-FGF and Astragalus membranaceus can induce the differentiation of rabbit bone marrow stromal cells into Schwan-like cells in vitro.
【學(xué)位授予單位】：廣西醫(yī)科大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2006
【分類(lèi)號(hào)】：R329.2

【參考文獻(xiàn)】

相關(guān)期刊論文 前3條

1 劉金保 ,董曉先 ,董燕湘 ,何慧華 ,董偉華 ,梁仲培 ,肖慶忠;多種中藥成分誘導(dǎo)大鼠骨髓間質(zhì)干細(xì)胞轉(zhuǎn)變?yōu)樯窠?jīng)元樣細(xì)胞[J];中國(guó)藥物與臨床;2003年03期

2 胡軍,劉小林,朱家愷,鄧宇斌,向劍平,易建華,原清濤;體外誘導(dǎo)獼猴骨髓基質(zhì)干細(xì)胞向雪旺細(xì)胞分化[J];中華創(chuàng)傷骨科雜志;2004年05期

3 董燕湘,董曉先,何慧華,劉金保;大鼠骨髓間質(zhì)干細(xì)胞用中藥絞股藍(lán)誘導(dǎo)為神經(jīng)細(xì)胞的研究[J];中華神經(jīng)科雜志;2003年05期

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本文編號(hào)：1647344