本文選題：骨髓間充質(zhì)干細(xì)胞　切入點(diǎn)：DAPI　出處：《重慶醫(yī)科大學(xué)》2006年碩士論文　論文類型：學(xué)位論文

【摘要】： 目的骨髓間充質(zhì)干細(xì)胞(Bone marrow mesenchymal stem cells, Bm-MSCs)具有高度增殖、自我更新能力,可向三個(gè)胚層的細(xì)胞分化[1],在機(jī)體組織損傷修復(fù)過程及細(xì)胞移植治療等多個(gè)領(lǐng)域有廣闊的應(yīng)用前景。目前,已有成功誘導(dǎo)Bm-MSCs分化為成骨細(xì)胞、軟骨細(xì)胞、脂肪細(xì)胞、神經(jīng)細(xì)胞、肝細(xì)胞、皮膚上皮細(xì)胞等的報(bào)道[2~7],尚未見腸壁結(jié)締組織影響B(tài)m-MSCs分化的研究。本實(shí)驗(yàn)擬①分離、培養(yǎng)、擴(kuò)增胎兒Bm-MSCs,觀察其生物學(xué)特性;②制備胎兒腸壁結(jié)締組織,遴選體外培養(yǎng)時(shí)間;③觀察胎兒腸壁結(jié)締組織能否誘導(dǎo)Bm-MSCs分化形成上皮細(xì)胞?EGF在胎兒腸壁結(jié)締組織誘導(dǎo)Bm-MSCs分化中的作用。為Bm-MSCs在消化管組織工程以及腸粘膜損傷性疾病治療研究中的應(yīng)用,提供基礎(chǔ)理論和實(shí)驗(yàn)依據(jù)。 第一部分 1材料與方法 1.1收集6例4~5個(gè)月引產(chǎn)胎兒的四肢骨髓,分離、培養(yǎng)、擴(kuò)增獲取Bm-MSCs。檢測Bm-MSCs的貼壁率、生長曲線;流式細(xì)胞儀檢測CD29、CD71、CD34及HLA-DR的表達(dá),以及細(xì)胞周期分析;細(xì)胞化學(xué)染色檢測非特異性酯酶、堿性磷酸酶及糖原。 1.2 DAPI標(biāo)記Bm-MSCs,不同時(shí)點(diǎn)熒光顯微鏡觀察,計(jì)算標(biāo)記率。
[Abstract]:Objective Bone marrow mesenchymal stem cells (Bm-MSCs) are highly proliferative, self-renewing, and can differentiate into three layers of cells. Bm-MSCs can be widely used in many fields, such as tissue damage repair and cell transplantation therapy, etc. At present, bone marrow mesenchymal stem cells (Bm-MSCs) can differentiate into three layers of cells. Bm-MSCs was successfully induced to differentiate into osteoblasts, chondrocytes, adipocytes, nerve cells, hepatocytes, skin epithelial cells and so on. Fetal Bm-MSCswere amplified to observe the biological characteristics of fetal intestinal wall connective tissue was prepared. The selection of culture time in vitro to observe whether fetal intestinal wall connective tissue can induce Bm-MSCs differentiation into epithelial cells. The role of EGF in the differentiation of Bm-MSCs induced by connective tissue of fetal intestinal wall provides theoretical and experimental basis for the application of Bm-MSCs in the study of digestive tract tissue engineering and the treatment of intestinal mucosal injury diseases. Part one. Materials and methods. 1.1 Bm-MSCswere collected from bone marrow of 6 fetuses of 4 ~ 5 months of induced labor. The adherent rate and growth curve of Bm-MSCs were detected by Bm-MSCs.The expression of CD29, CD71, CD34 and HLA-DR, and cell cycle analysis were detected by flow cytometry. Nonspecific esterase, alkaline phosphatase and glycogen were detected by cytochemical staining. 1. 2 DAPI labeled Bm-MSCs, fluorescence microscope at different time points, the labeling rate was calculated.
【學(xué)位授予單位】：重慶醫(yī)科大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2006
【分類號(hào)】：R321

【引證文獻(xiàn)】

相關(guān)期刊論文 前1條

1 宋祥福;孫冰雪;張靜春;龔守良;;同種異體大鼠骨髓間充質(zhì)干細(xì)胞移植對(duì)放射性空腸損傷的修復(fù)作用[J];中國比較醫(yī)學(xué)雜志;2011年08期

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本文編號(hào)：1632632