本文選題：流行性感冒病毒　切入點：Vero細胞　出處：《吉林大學》2007年碩士論文　論文類型：學位論文

【摘要】： 流感是由甲(A)、乙(B)、丙(C)三型流感病毒分別引起的一種急性呼吸道傳染病。甲、乙、丙不僅反映了病毒被發(fā)現(xiàn)的年代及前后順序,更主要的是反映了對人類危害程度的順序。其重要性在于可引起嚴重的發(fā)病率和死亡率,影響學校、公共服務機構和各單位的正常工作。超額死亡率也與流感有關,死亡者多為患有慢性疾病和年邁體衰的老年人。目前唯一可行的流感預防措施是疫苗接種。 自1941年流感滅活疫苗在美國首次批準使用以來,流感疫苗已使用60多年。在此期間,廣泛的經(jīng)驗已證明疫苗的安全性和效力。每年在流感流行季節(jié)前在流感的高危人群中進行預防接種是減輕流感危害的重要措施。疫苗接種不僅可以降低高危人群的發(fā)病率和死亡率,也可減輕癥狀,降低并發(fā)癥的發(fā)生,降低繼續(xù)傳播的機率。 目前研究應用于滅活流感疫苗生產(chǎn)的細胞系主要有MDCK、VERO、PER.C6三個細胞系。其中Vero傳代細胞系解決了外源病毒和致癌性的問題,且易于大規(guī)模培養(yǎng),被WHO推薦作為疫苗生產(chǎn)細胞基質(zhì)之一,已成功開發(fā)出了狂犬病、脊髓灰質(zhì)炎、流行性乙腦炎等疫苗。使用哺乳動物細胞培養(yǎng)技術替代雞胚來生產(chǎn)流感疫苗將是大勢所趨。 基于上述情況,本文作者通過廣泛的文獻檢索與反復試驗,現(xiàn)已完成各(亞)型流感病毒于Vero細胞內(nèi)的快速傳代工藝,建立了流感病毒滅活疫苗(Vero細胞)生產(chǎn)用病毒種子批系統(tǒng),解決了流感病毒于細胞內(nèi)傳代穩(wěn)定性問題,為進一步研制流感病毒滅活疫苗(Vero細胞)打下了良好的基礎。
[Abstract]:Influenza is an acute respiratory infectious disease caused by A, B, C) influenza viruses. A, B, C not only reflect the date and sequence of the virus being discovered. More importantly, it reflects the order of harm to human beings. Its importance lies in causing serious morbidity and mortality, affecting the normal work of schools, public services and units. Excess mortality is also associated with influenza. Most of the deaths are among the elderly with chronic illness and frailty. Vaccination is the only available influenza prevention measure. The flu vaccine has been in use for more than 60 years since 1941, when it was first approved for use in the United States. Extensive experience has proved the safety and effectiveness of vaccines. Vaccination every year before the influenza epidemic season in the influenza high-risk population is an important measure to reduce the risk of influenza. Vaccination can not only reduce the high-risk population. Morbidity and mortality, Can also reduce symptoms, reduce the incidence of complications, reduce the probability of further transmission. At present, the main cell lines used in the production of inactivated influenza vaccine are MDCK VEROPER.C6. Among them, Vero subculture cell line solves the problems of exogenous virus and carcinogenicity, and is easy to be cultured on a large scale. Rabies, poliomyelitis and Japanese encephalitis have been successfully developed as one of the cell matrix for vaccine production by WHO. It is a general trend to use mammalian cell culture technology to replace chicken embryo to produce influenza vaccine. In view of the above situation, the author has completed the rapid passage of various (subtype) influenza viruses in Vero cells through extensive literature retrieval and repeated experiments. A virus seed batch system was established for the production of inactivated influenza virus vaccine (Vero cells), which solved the problem of intracellular transmission stability of influenza virus and laid a good foundation for the further development of inactivated influenza virus vaccine Vero cells.
【學位授予單位】：吉林大學
【學位級別】：碩士
【學位授予年份】：2007
【分類號】：R392

【共引文獻】

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相關碩士學位論文 前6條

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