本文關(guān)鍵詞： 毛發(fā)生長(zhǎng)發(fā)育異常 動(dòng)物模型 ICR小鼠 C57BL/6小鼠 基因芯片 基因表達(dá)譜　出處：《第二軍醫(yī)大學(xué)》2006年博士論文　論文類型：學(xué)位論文

【摘要】：本研究從給藥劑量、給藥時(shí)間、性狀有無(wú)遺傳性幾個(gè)方面進(jìn)行研究,建立了兩種品系小鼠毛發(fā)生長(zhǎng)發(fā)育異常的動(dòng)物模型。并從仔鼠毛發(fā)的生長(zhǎng)及分布情況、毛發(fā)類型、毛發(fā)長(zhǎng)度、毛發(fā)直徑及毛發(fā)密度幾個(gè)方面對(duì)各組仔鼠的毛發(fā)性狀進(jìn)行了描述。10mg/kg組仔鼠在給藥期肉眼未見毛發(fā)生長(zhǎng)。在此基礎(chǔ)上,用小鼠全基因組芯片對(duì)接觸藥物組仔鼠及對(duì)照組仔鼠皮膚毛囊組織的基因表達(dá)譜進(jìn)行了分析,發(fā)現(xiàn)大部分編碼構(gòu)成毛發(fā)的蛋白質(zhì)的相應(yīng)基因(Krt及Krtap)在給藥組仔鼠的皮膚毛囊組織中的表達(dá)明顯下調(diào),而且參與Krt及Krtap表達(dá)調(diào)控的基因也發(fā)生了明顯變化。在此基礎(chǔ)上對(duì)CK-794誘發(fā)的毛發(fā)生長(zhǎng)發(fā)育異常可能的機(jī)制進(jìn)行初步分析。
[Abstract]:In this study, animal models of abnormal hair growth and development in two strains of mice were established from the aspects of dosage, time and heredity of administration, and the growth and distribution of hair and hair types in two strains of mice were also studied. Hair length, hair diameter and hair density were used to describe the hair characteristics of rats in each group. 10 mg / kg group had no hair growth in the naked eye during the administration period. The gene expression profiles of the skin hair follicles of the mice exposed to the drug and the control group were analyzed by using the whole genome microarray of mice. It was found that the expression of most of the corresponding genes, Krt and Krtapin, which encodes the proteins that constitute hair, was significantly down-regulated in the skin hair follicles of the rats in the administration group. Moreover, the genes involved in the regulation of Krt and Krtap expression also changed obviously. The possible mechanism of hair growth and development abnormality induced by CK-794 was analyzed.
【學(xué)位授予單位】：第二軍醫(yī)大學(xué)
【學(xué)位級(jí)別】：博士
【學(xué)位授予年份】：2006
【分類號(hào)】：R-332

【共引文獻(xiàn)】

相關(guān)碩士學(xué)位論文 前1條

1 韓勇;不同培養(yǎng)基、藥物對(duì)體外人游離毛囊生長(zhǎng)的影響[D];揚(yáng)州大學(xué);2007年

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本文編號(hào)：1553501