本文關(guān)鍵詞： 貓 小腦皮質(zhì) 衰老 Nissl染色 免疫組織化學(xué) 膠質(zhì)纖維酸性蛋白 神經(jīng)絲蛋白 S100蛋白 浦肯野細(xì)胞 超微結(jié)構(gòu)　出處：《安徽師范大學(xué)》2006年碩士論文　論文類(lèi)型：學(xué)位論文

【摘要】： 小腦是調(diào)節(jié)軀體運(yùn)動(dòng)的高級(jí)中樞,主要接受與運(yùn)動(dòng)有關(guān)的信息,包括本體感覺(jué)信息和部分外周感覺(jué)信息,這些與運(yùn)動(dòng)有關(guān)的信息匯聚到小腦浦肯野細(xì)胞(PC),而PC的活動(dòng)又由其軸突通過(guò)小腦核作用到脊髓、腦干及大腦皮層。衰老造成小腦皮質(zhì)的形態(tài)結(jié)構(gòu)、細(xì)胞構(gòu)筑及神經(jīng)遞質(zhì)等方面的改變,可能導(dǎo)致老年人和動(dòng)物小腦功能衰退。貓是一類(lèi)比較高級(jí)的哺乳動(dòng)物,衰老過(guò)程中,貓中樞神經(jīng)系統(tǒng)結(jié)構(gòu)和功能改變已有廣泛研究,但其小腦皮質(zhì)年齡相關(guān)的形態(tài)學(xué)變化未見(jiàn)報(bào)道。本文對(duì)青年貓和老年貓小腦皮質(zhì)結(jié)構(gòu)的差異進(jìn)行比較研究,以期為探討老年小腦功能衰退的神經(jīng)機(jī)制積累形態(tài)學(xué)資料。 1.以青年貓(1-3齡)和老年貓(12-13齡)小腦皮質(zhì)為研究對(duì)像,運(yùn)用Nissl染色顯示小腦皮質(zhì)神經(jīng)元及皮質(zhì)分層結(jié)構(gòu),免疫組織化學(xué)ABC法顯示星形膠質(zhì)細(xì)胞特異性標(biāo)志物膠質(zhì)纖維酸性蛋白免疫陽(yáng)性(Glial fibrillary acidic protein immunoreactive,GFAP-IR)星形膠質(zhì)細(xì)胞和神經(jīng)絲蛋白免疫陽(yáng)性(Neurofilament immunoreactive,NF-IR)神經(jīng)元。與青年貓相比,老年貓小腦皮質(zhì)總厚度及分子層厚度顯著下降,顆粒層厚度明顯增加;各層神經(jīng)元密度明顯降低;PC胞體收縮,樹(shù)突分枝大量丟失;顆粒層中GFAP-IR細(xì)胞密度顯著增大,陽(yáng)性反應(yīng)增強(qiáng)。推測(cè)衰老過(guò)程中小腦皮質(zhì)神經(jīng)元丟失,PC樹(shù)突野萎縮,可能是導(dǎo)致小腦皮質(zhì)接受和整合信息功能退化的重要原因之一;老年個(gè)體小腦皮質(zhì)中星形膠質(zhì)細(xì)胞活動(dòng)增強(qiáng)對(duì)皮質(zhì)神經(jīng)元可能起保護(hù)作用。 2.運(yùn)用電鏡技術(shù)觀察青年貓和老年貓小腦PC的超微結(jié)構(gòu)。結(jié)果顯
[Abstract]:The cerebellum is the advanced center that regulates the body movement. It mainly receives the information related to movement, including proprioceptive information and some peripheral sensory information. This motion-related information is gathered into the Purkinje cells of the cerebellum, and the activity of PC is activated by its axons through the cerebellar nucleus to the spinal cord, the brainstem and the cerebral cortex. Aging results in the morphology of the cerebellar cortex. Changes in cellular architecture and neurotransmitters may lead to the decline of cerebellar function in the elderly and animals. However, the age-related morphological changes of cerebellar cortex were not reported. The differences of cerebellar cortex structure between young cats and old cats were studied in order to accumulate morphological data for exploring the neuromechanism of cerebellar function decline in elderly cats. 1. The cerebellar cortex of young cats (1-3 years old) and old cats (12-13 years old) were studied. The cerebellar cortical neurons and cortical stratified structure were demonstrated by Nissl staining. Immunohistochemical ABC method showed astrocytes and neurofilaments immunoreactive NF-IRN neurons in astrocytes, glial fibrillary acidic protein immunoreactive protein, glial fibrillary acidic protein (GFAP-IRI) and neurofilaments immunoreactive (NF-IRR) neurons in young cats. The total thickness of cerebellar cortex and the thickness of molecular layer decreased significantly, the thickness of granular layer increased significantly, the density of neurons in each layer decreased the contraction of PC cell body and the loss of dendritic branches, and the density of GFAP-IR cells in granular layer increased significantly. It is speculated that the loss of cerebellar cortical neurons and atrophy of PC dendritic field during senescence may be one of the important reasons for the degeneration of cerebellar cortex reception and integration information function. Enhanced astrocyte activity in the cerebellar cortex may protect cortical neurons in the elderly. 2. The ultrastructure of cerebellar PC in young and old cats was observed by electron microscope.
【學(xué)位授予單位】：安徽師范大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2006
【分類(lèi)號(hào)】：R33

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