本文關(guān)鍵詞： 金黃色葡萄球菌 組氨酸激酶AgrC 脂質(zhì)體 蛋白脂質(zhì)體 膜蛋白　出處：《高等學(xué)校化學(xué)學(xué)報(bào)》2014年09期 　論文類型：期刊論文

【摘要】：通過改變脂質(zhì)體中磷脂成分,構(gòu)建了不同電性的脂質(zhì)體.利用表面活性劑介導(dǎo)方法,將截短的金黃色葡萄球菌細(xì)胞膜上的組氨酸激酶AgrC(AgrCTM6-7C)蛋白重構(gòu)到不同電性的脂質(zhì)體上.結(jié)果表明,陰離子脂質(zhì)體對AgrCTM6-7C蛋白的鑲嵌效率明顯高于陽離子脂質(zhì)體,約60%~70%鑲嵌至陰離子脂質(zhì)體中的AgrCTM6-7C蛋白的細(xì)胞質(zhì)域朝向脂質(zhì)體囊泡的外部,并保持較高活性.利用圓二色光譜比較了AgrCTM6-7C蛋白在表面活性劑膠束和脂質(zhì)體中的二級結(jié)構(gòu)穩(wěn)定性,發(fā)現(xiàn)陰離子脂質(zhì)體對AgrCTM6-7C蛋白的二級結(jié)構(gòu)具有一定的保護(hù)作用,可明顯提高蛋白的熱穩(wěn)定性.
[Abstract]:Different electrical liposomes were constructed by changing the phospholipid composition of liposomes. The short staphylococcus aureus membrane histidine kinase AgrCon AgrCTM6-7C protein was reconstructed into different electrical liposomes. The inlay efficiency of anionic liposomes to AgrCTM6-7C protein was significantly higher than that of cationic liposomes. About 70% of the cytoplasmic domain of the AgrCTM6-7C protein embedded in the anion liposome is oriented towards the outside of the liposome vesicle. The secondary structure stability of AgrCTM6-7C protein in surfactant micelles and liposomes was compared by circular dichroism spectroscopy. It was found that anionic liposomes had a certain protective effect on the secondary structure of AgrCTM6-7C protein and could obviously improve the thermal stability of the protein.
【作者單位】： 大連民族學(xué)院生物技術(shù)與資源利用國家民委-教育部重點(diǎn)實(shí)驗(yàn)室;大連民族學(xué)院生命科學(xué)學(xué)院;中國科學(xué)院大連化學(xué)物理研究所;大連工業(yè)大學(xué)生物工程學(xué)院;
【基金】：國家自然科學(xué)基金(批準(zhǔn)號:21272031) 大連民族學(xué)院研究生創(chuàng)新基金項(xiàng)目(批準(zhǔn)號:YCX2013016)資助~~
【分類號】：R378.1;O629.73
【正文快照】： 金黃色葡萄球菌是一種重要的人類病原菌,可引起肺炎、敗血癥等許多嚴(yán)重感染[1~4].金黃色葡萄球菌的附屬基因調(diào)節(jié)(agr)系統(tǒng)是其重要基因表達(dá)調(diào)控系統(tǒng),對細(xì)菌的毒力調(diào)節(jié)起到關(guān)鍵作用[5~7].該系統(tǒng)包含膜受體激酶AgrC和反應(yīng)調(diào)節(jié)蛋白AgrA,二者共同構(gòu)成經(jīng)典的雙組分信號轉(zhuǎn)導(dǎo)系統(tǒng)[8].，

本文編號：1474500