本文關(guān)鍵詞：從臍血單個核細胞誘導(dǎo)巨核細胞體外擴增及分化的實驗研究　出處：《福建醫(yī)科大學(xué)》2005年碩士論文　論文類型：學(xué)位論文

  更多相關(guān)文章： 臍血 巨核細胞 細胞因子 體外擴增 誘導(dǎo)分化

【摘要】：巨核細胞是血小板的前體細胞。將部分臍血進行巨核系祖細胞體外擴增后再輸注給患者,是有望解決臍血造血干細胞移植后患者血小板恢復(fù)遲緩這一難題的有效途徑,但目前最佳擴增條件的確立仍處于實驗階段。本項研究從臍血單個核(MNC)出發(fā),在無血清培養(yǎng)體系中比較了TPO、TPO+SCF、TPO+SCGF、TPO+IL-3、TPO+EPO5 種簡單細胞因子組合對刺激巨核細胞擴增的作用,經(jīng)體外培養(yǎng)14 天后,TPO+SCF 組CD41~+細胞比例和擴增倍數(shù)最高,分別為20.95±2.83%和48.40±5.71倍;接著,將TPO 組和TPO+SCF 組的臍血MNC 均培養(yǎng)到18 天,以觀察培養(yǎng)后期(9d、14d、18d)巨核細胞的凋亡情況,結(jié)果發(fā)現(xiàn)TPO+SCF 組的MNC 和CD41~+細胞的凋亡率與單用TPO 組相比均有顯著差異(P0.05);最后,以TPO+SCF組為研究對象,采用RT-PCR 方法檢測了轉(zhuǎn)錄因子GATA-1、NF-E2 和系特異抗原GPⅡb mRNA 表達情況,發(fā)現(xiàn)培養(yǎng)14 天時三者的表達均達到高峰。上述結(jié)果證明:SCF 和TPO 的協(xié)同刺激在有效促進巨核細胞的擴增的同時,也在有效誘導(dǎo)其分化。
[Abstract]:Megakaryocytes are progenitor cells of platelets. Some umbilical cord blood was amplified by megakaryocyte progenitor cells in vitro and then infused to patients. It is an effective way to solve the problem of delayed platelet recovery after cord blood hematopoietic stem cell transplantation. However, the optimal amplification conditions are still in the experimental stage. This study compared TPO SCF in serum-free culture system from cord blood mononuclear cells (MNCs). The effect of simple cytokine combination of TPO SCGF- TPO IL-3 TPO EPO5 on the proliferation of megakaryocytes cultured in vitro for 14 days. CD41 ~ ~ cells in TPO SCF group were 20.95 鹵2.83% and 48.40 鹵5.71 times respectively. Then, the cord blood MNC of TPO group and TPO SCF group were cultured for 18 days to observe the apoptosis of megakaryocyte in the late stage of culture. The results showed that the apoptosis rates of MNC and CD41 ~ ~ cells in TPO SCF group were significantly different from those in TPO group. Finally, the expression of transcription factor GATA-1NF-E2 and lineage-specific antigen GP 鈪，

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