本文關(guān)鍵詞：心肌細(xì)胞培養(yǎng)純化與小鼠骨髓間充質(zhì)干細(xì)胞分化誘導(dǎo)的研究　出處：《佳木斯大學(xué)》2007年碩士論文　論文類型：學(xué)位論文

  更多相關(guān)文章： 心肌細(xì)胞 培養(yǎng) 骨髓間充質(zhì)干細(xì)胞 誘導(dǎo) 分化

【摘要】： 目的:探討心肌細(xì)胞分離、純化和培養(yǎng)方法;研究骨髓間充質(zhì)干細(xì)胞(BMSCs)在不同心肌細(xì)胞微環(huán)境作用下,向心肌樣細(xì)胞分化能力。 方法:采用不同時間點,通過差速貼壁純化、培養(yǎng)新生乳鼠心肌細(xì)胞。將原代乳鼠心肌細(xì)胞與BMSCs聯(lián)合培養(yǎng),分為:接觸性聯(lián)合培養(yǎng)組,將心肌細(xì)胞與BMSCs按2㑳1的比例種植在同一培養(yǎng)皿中;非接觸性聯(lián)合培養(yǎng)組,將分別種植有心肌細(xì)胞、BMSCs的蓋玻片按2㑳1的比例種放入同一培養(yǎng)皿中共同培養(yǎng);對照組,BMSCs常規(guī)培養(yǎng)。二周后檢測各實驗組細(xì)胞中,心肌特異性蛋白肌鈣蛋白T(cTnT)表達(dá)情況。 結(jié)果:心肌細(xì)胞培養(yǎng),采用差速貼壁60min組與90min組之間有統(tǒng)計學(xué)意義,差速貼壁60min組、90min組與差速貼壁120min組兩兩之間有統(tǒng)計學(xué)差別。免疫細(xì)胞化學(xué)鑒定:(1)接觸性聯(lián)合培養(yǎng)組細(xì)胞均表達(dá)肌鈣蛋白T(cTnT);(2)非接觸性聯(lián)合培養(yǎng)組細(xì)胞均表達(dá)肌鈣蛋白T(cTnT);(3)對照組細(xì)胞不表達(dá)肌鈣蛋白T(cTnT)。 結(jié)論:心肌細(xì)胞培養(yǎng)過程中,采用差速貼壁60min較差速貼壁90min、120min獲得的心肌細(xì)胞數(shù)量高;接觸性聯(lián)合培養(yǎng)組與非接觸性聯(lián)合培養(yǎng)組均可將BMSCs誘導(dǎo)分化為心肌樣細(xì)胞,對照組BMSCs無分化。
[Abstract]:Aim: to investigate the methods of isolation, purification and culture of cardiomyocytes, and to study the ability of bone marrow mesenchymal stem cells (BMSCs) to differentiate into cardiomyocyte-like cells under different microenvironment. Methods: neonatal neonatal rat cardiomyocytes were cultured at different time points by differential adherent purification. Primary neonatal rat cardiomyocytes were co-cultured with BMSCs and divided into contact co-culture group. The relationship between cardiomyocytes and BMSCs was 2? 1% was planted in the same culture dish, and in the non-contact co-culture group, the cover glass of BMSCs implanted with cardiomyocytes was 2? (1) the proportion of BMSCs was cultured in the same culture dish, and the control group BMSCs were cultured routinely. Two weeks later, the expression of cardiac troponin TnT was detected in the cells of each experimental group. Results: there was significant difference between 60 min and 90 min groups in the culture of cardiomyocytes. There was significant difference between 90 min group and 120 min differential adhesion group. (2) the cells in the non-contact co-culture group all expressed troponin TnTnT; 3) the control group did not express troponin TnTnT. Conclusion: in the process of cardiomyocyte culture, the number of cardiomyocytes obtained by differential adhesion for 60 min or 90 min or 120 min is high. Contact co-culture group and non-contact co-culture group could induce BMSCs to differentiate into cardiomyocyte-like cells, while BMSCs in control group had no differentiation.
【學(xué)位授予單位】：佳木斯大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2007
【分類號】：R329

【引證文獻(xiàn)】

相關(guān)碩士學(xué)位論文 前2條

1 和小娥;兔骨髓間充質(zhì)干細(xì)胞分離培養(yǎng)及定向分化的研究[D];河南農(nóng)業(yè)大學(xué);2011年

2 閆穎穎;兔骨髓間充質(zhì)干細(xì)胞向心肌樣細(xì)胞的體外誘導(dǎo)分化研究[D];西北農(nóng)林科技大學(xué);2010年

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