【摘要】：將囊液抗原蛋白采用雙向凝膠電泳分離,經(jīng)考馬斯亮藍(lán)染色后用雙向電泳圖像分析軟件分析蛋白點(diǎn),以腦囊尾蚴病患者血清為一抗進(jìn)行蛋白質(zhì)印跡分析(Western blotting),選取陽(yáng)性蛋白點(diǎn)進(jìn)行質(zhì)譜分析,獲取肽質(zhì)量指紋圖譜,采用Mascot軟件在NCBI中搜尋、比對(duì),篩選出抗豬囊尾蚴IgG4特異性抗原。囊液抗原經(jīng)雙向電泳檢測(cè)到(201±5)個(gè)蛋白斑點(diǎn),相對(duì)分子質(zhì)量(M_r)為10 000~170 000,等電點(diǎn)(pI)為3.0~10.0。Western blotting分析顯示,與患者血清中特異IgG4抗體結(jié)合的抗原以小分子量蛋白為主,從中篩選出51個(gè)(抗原)點(diǎn)進(jìn)行質(zhì)譜分析,其中有21個(gè)點(diǎn)峰值大于39。對(duì)比分析顯示,高峰值點(diǎn)與10種囊尾蚴蛋白有關(guān),其中最為密切相關(guān)的為4種蛋白,按分值高低排序分別為T(mén)s8B2、Ts8B3、10ku抗原和Sequence 3。
[Abstract]:The antigen protein of cysticercosis was separated by two-dimensional gel electrophoresis. After staining with Coomassie brilliant blue, the protein spots were analyzed by two-dimensional electrophoresis image analysis software. (Western blotting), positive protein spots were selected for mass spectrometry with serum of patients with cerebral cysticercosis as primary antibody for mass spectrometry analysis. The peptide mass fingerprint was obtained. Mascot software was used to search for IgG4 specific antigens against cysticercus cellulosae. The antigen of capsule fluid was detected by two-dimensional electrophoresis with (201 鹵5) protein spots, the relative molecular weight (M 鈮，

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