發(fā)布時(shí)間：2019-05-14 22:33

【摘要】：目的:探討脛骨內(nèi)注射法建立的大鼠乳腺癌骨轉(zhuǎn)移疼痛模型骨病灶的影像學(xué)確認(rèn)方法。方法:將Walker 256大鼠乳腺癌腹水瘤注射入Wistar大鼠脛骨腔內(nèi),制作乳腺癌骨癌痛模型;以脛骨內(nèi)注射等量PBS溶液作為對(duì)照組。使用von-Frey法,測量大鼠后足機(jī)械痛覺閾值,觀察各組疼痛變化。對(duì)模型組和對(duì)照組進(jìn)行~(99mTc)-MDP骨顯像和CT成像,觀察骨病灶的骨代謝變化及骨質(zhì)結(jié)構(gòu)的變化。結(jié)果:造模的第3~5天,乳腺癌骨轉(zhuǎn)移疼痛大鼠的機(jī)械痛覺閾值較基礎(chǔ)值明顯下降(P0.01),并持續(xù)下降83.2%左右,至第9天左右達(dá)到最痛(P0.001),后下降曲線趨于緩慢變化。造模3~5天后,模型組機(jī)械痛覺閾值顯著低于對(duì)照組(P0.01),這個(gè)差別持續(xù)增大至第9天左右。~(99mTc)-MDP骨顯像顯示所有模型組大鼠脛骨種植腫瘤細(xì)胞處放射性攝取顯著高于對(duì)側(cè),CT見造模側(cè)脛骨上段骨質(zhì)破壞,對(duì)側(cè)骨質(zhì)結(jié)構(gòu)正常,未見其他部位骨病灶存在。而對(duì)照組所有大鼠骨顯像示兩側(cè)脛骨放射性分布對(duì)稱,CT顯示兩側(cè)骨質(zhì)結(jié)構(gòu)正常。結(jié)論:脛骨內(nèi)注射法大鼠骨癌痛模型疼痛確切。~(99mTc)-MDP骨顯像以及CT可以從骨代謝和骨結(jié)構(gòu)評(píng)估骨癌痛模型病灶的發(fā)生,是確認(rèn)此類骨轉(zhuǎn)移疼痛模型造模的成功較好的影像學(xué)方法。
[Abstract]:Objective: to investigate the imaging confirmation of bone lesions in rat breast cancer bone metastasis pain model established by intra-tibia injection. Methods: Walker 256 rats were injected into the tibia cavity of Wistar rats to establish the model of breast cancer pain, and the same amount of PBS solution was injected into the tibia as the control group. The mechanical pain threshold of posterior foot of rats was measured by von-Frey method, and the changes of pain in each group were observed. ~ (99mTc)-MDP bone imaging and CT imaging were performed in the model group and the control group to observe the changes of bone metabolism and bone structure. Results: on the 3rd to 5th day, the mechanical pain threshold of rats with bone metastasis pain of breast cancer was significantly lower than that of the basic value (P0.01), and continued to decrease by 83.2%, reaching the highest pain on the 9th day (P0.001). The post-decline curve tends to change slowly. After 5 days of modeling, the threshold of mechanical pain in the model group was significantly lower than that in the control group (P 0.01). The difference continued to increase until the 9th day. ~ (99mTc)-MDP bone imaging showed that the radioactivity uptake of tumor cells in the tibia of all model groups was significantly higher than that of the contralateral side. CT showed bone destruction in the upper tibia of the model side, and the contralateral bone structure was normal. No bone lesions were found in other parts. In the control group, the radioactivity distribution of both tibia was symmetrical on bone imaging, and the bone structure of both sides was normal on CT. Conclusion: the pain of bone cancer pain model in rats by intra-tibia injection is accurate. ~ (99mTc)-MDP bone imaging and CT can evaluate the occurrence of bone cancer pain model from bone metabolism and bone structure. It is a good imaging method to confirm the success of this kind of bone metastasis pain model.
【作者單位】： 復(fù)旦大學(xué)附屬華山醫(yī)院核醫(yī)學(xué)科;同濟(jì)大學(xué)附屬東方醫(yī)院核醫(yī)學(xué)科;復(fù)旦大學(xué)上海醫(yī)學(xué)院中西醫(yī)結(jié)合系;
【基金】：國家自然科學(xué)基金No.81201113 上海市衛(wèi)計(jì)委青年科學(xué)基金項(xiàng)目(20124Y069)~~
【分類號(hào)】：R-332;R737.9;R738.1

【參考文獻(xiàn)】

相關(guān)期刊論文 前6條

1 韓帥;楊興海;萬宗淼;周旺;姜東杰;尹華斌;王霆;肖建如;;用于活體成像的人肺腺癌自發(fā)骨轉(zhuǎn)移小鼠模型[J];國際骨科學(xué)雜志;2015年02期

2 余家華;李秋林;鄭榮壽;張思維;葛蓮英;曹驥;陳萬青;;中國2009年喉惡性腫瘤發(fā)病和死亡分析[J];中國腫瘤;2013年05期

3 黃章翔;李曉青;趙志奇;王妙苗;繆雪蓉;俞衛(wèi)鋒;楊立群;;癌癥痛大鼠模型中鏡像痛現(xiàn)象觀察[J];西南國防醫(yī)藥;2009年01期

4 ;The pinhole SPECT for animal model of bone metastasis with SPC-A-1BM human pulmonary adenocarcinoma bone metastasis cell line[J];Nuclear Science and Techniques;2008年05期

5 李曉青;孫玉明;黃章翔;王妙苗;吳鏡湘;繆雪蓉;徐愛民;朱忠正;趙志奇;俞衛(wèi)鋒;;Walker256乳腺癌細(xì)胞構(gòu)建大鼠脛骨骨癌痛模型[J];中國腫瘤生物治療雜志;2008年01期

6 譚煌英,于莉莉,高福云,崔建,潘琳,田鑫,程志強(qiáng),賈立群,李佩文;大鼠骨癌痛模型的建立及組織學(xué)研究[J];癌癥進(jìn)展;2005年01期

【共引文獻(xiàn)】

相關(guān)期刊論文 前10條

1 劉從進(jìn);宋潔平;毛應(yīng)啟梁;王淵愷;朱匯慶;張光明;劉興黨;;脛骨內(nèi)注射大鼠骨轉(zhuǎn)移疼痛模型的顯像研究[J];中國醫(yī)學(xué)計(jì)算機(jī)成像雜志;2016年03期

2 吳紅杰;鄒小明;;結(jié)直腸癌術(shù)后吻合口瘺的診治及預(yù)防[J];中華結(jié)直腸疾病電子雜志;2016年02期

3 韓曉東;劉擘;田延鋒;李芳;趙增仁;翟從R，

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