【摘要】：自然界中生物常常面臨氮資源限制,它們可能會采用不同的策略來適應.通過比較現(xiàn)存物種的研究認為生物采取的是節(jié)約氮的策略,而進化實驗的研究發(fā)現(xiàn)生物提高了吸收氮資源的能力.這2類研究都有一些方法缺陷,因此本研究設計了一個更系統(tǒng)的進化實驗,讓大腸桿菌種群在2種氮資源環(huán)境中進化1 300代,分析其對不同氮資源環(huán)境的適應性進化.1 300代后,2種氮資源環(huán)境中進化的大腸桿菌株系適合度均提高,低氮進化株系適合度提升幅度大于高氮進化株系.與祖先相比,低氮株系氮質(zhì)量分數(shù)和DNA質(zhì)量分數(shù)均顯著提高,碳、氮質(zhì)量比顯著降低,而高氮株系在RNA質(zhì)量分數(shù)上顯著提高.說明在本研究體系中,大腸桿菌采用了增強氮獲取能力的策略來適應氮限制,而不是節(jié)約氮.
[Abstract]:Living things in nature often face nitrogen resource constraints, and they may adopt different strategies to adapt. Compared with the existing species, it is believed that organisms adopt the strategy of saving nitrogen, while evolutionary experiments show that organisms improve their ability to absorb nitrogen resources. These two types of studies have some methodological defects, so this study designed a more systematic evolutionary experiment to let the Escherichia coli population evolve 1,300 generations in two nitrogen resource environments. The adaptive evolution of these strains to different nitrogen resources and environments was analyzed. 1 300 generations later, the fitness of the two strains was improved, and that of the low nitrogen evolutionary strain was greater than that of the high nitrogen evolutionary strain. Compared with their ancestors, the nitrogen mass fraction and DNA mass fraction of low nitrogen plant lines were significantly increased, while the mass ratio of carbon to nitrogen was significantly decreased, while the mass fraction of RNA of high nitrogen lines was significantly increased. In this study, E. coli used the strategy of enhancing the ability of nitrogen acquisition to adapt to nitrogen limitation, rather than saving nitrogen.
【作者單位】： 北京師范大學生物多樣性與生態(tài)工程教育部重點實驗室;
【基金】：國家自然科學基金資助項目(31030014)
【分類號】：R378

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