費城染色體陽性急性淋巴細胞白血病小鼠模型的建立和鑒定
發(fā)布時間：2019-01-30 15:36
【摘要】：目的:建立Ph~+急性淋巴細胞白血病(ALL)小鼠模型,為Ph~+ALL研究提供工具。方法:用M ig190逆轉錄病毒轉染BABL/c小鼠骨髓細胞產生CML樣小鼠,分選CML樣小鼠BCR-ABL~+B細胞,輸注同系小鼠建立Ph~+ALL模型;應用流式細胞術鑒定免疫表型,RT-PCR和Western blot鑒定BCR-ABL轉錄和蛋白表達。結果:M ig190逆轉錄病毒轉染后小鼠迅速發(fā)生CM L樣病變,輸注來自CM L樣小鼠的BCR-ABL~+B細胞后受鼠均發(fā)生急性淋巴細胞白血病,免疫分型顯示白血病細胞CD19~+;RT-PCR和Western Blot均檢測到BCR-ABL,符合Ph~+ALL的免疫表型和分子生物學特征。結論:成功開發(fā)了一種新型Ph~+ALL小鼠模型的建立方法,能夠為研究Ph~+ALL提供一有力工具。
[Abstract]:Objective: to establish Ph~ acute lymphoblastic leukemia (Ph~) (ALL) mouse model and to provide a tool for the study of Ph~ ALL. Methods: M ig190 retrovirus was transfected into the bone marrow cells of BABL/c mice to produce CML like mice. BCR-ABL~ B cells of CML like mice were selected and the Ph~ ALL model was established in the same strain mice. Flow cytometry was used to identify the immunophenotype, and RT-PCR and Western blot were used to identify the transcription and protein expression of BCR-ABL. Results: M ig190 retrovirus-transfected mice developed CM L-like lesions rapidly, and acute lymphoblastic leukemia (ALL) occurred in all recipient mice after transfection of BCR-ABL~ B cells from CM L-like mice. Both RT-PCR and Western Blot detected BCR-ABL, in accordance with the immunophenotype and molecular biological characteristics of Ph~ ALL. Conclusion: a new Ph~ ALL mouse model was successfully developed, which can provide a powerful tool for the study of Ph~ ALL.
【作者單位】： 徐州醫(yī)科大學血液病研究所;徐州醫(yī)科大學附屬醫(yī)院血液科;
【基金】：國家自然科學基金(81471580、81272206、81300399) 江蘇省普通高校研究生科研創(chuàng)新計劃(SJEE14-0199)
【分類號】：R733.71;R-332
[Abstract]:Objective: to establish Ph~ acute lymphoblastic leukemia (Ph~) (ALL) mouse model and to provide a tool for the study of Ph~ ALL. Methods: M ig190 retrovirus was transfected into the bone marrow cells of BABL/c mice to produce CML like mice. BCR-ABL~ B cells of CML like mice were selected and the Ph~ ALL model was established in the same strain mice. Flow cytometry was used to identify the immunophenotype, and RT-PCR and Western blot were used to identify the transcription and protein expression of BCR-ABL. Results: M ig190 retrovirus-transfected mice developed CM L-like lesions rapidly, and acute lymphoblastic leukemia (ALL) occurred in all recipient mice after transfection of BCR-ABL~ B cells from CM L-like mice. Both RT-PCR and Western Blot detected BCR-ABL, in accordance with the immunophenotype and molecular biological characteristics of Ph~ ALL. Conclusion: a new Ph~ ALL mouse model was successfully developed, which can provide a powerful tool for the study of Ph~ ALL.
【作者單位】： 徐州醫(yī)科大學血液病研究所;徐州醫(yī)科大學附屬醫(yī)院血液科;
【基金】：國家自然科學基金(81471580、81272206、81300399) 江蘇省普通高校研究生科研創(chuàng)新計劃(SJEE14-0199)
【分類號】：R733.71;R-332
【相似文獻】
相關期刊論文 前10條
1 王珍祥,吳軍,易紹萱,李世榮;反義及正義α-平滑肌肌動蛋白逆轉錄病毒載體的構建與表達[J];中國美容醫(yī)學;2003年03期
2 楊國紅;姬文婕;周欣;畢瑩;李玉明;;重組大鼠ΔNΔC/VEGF-C/Cys152Ser逆轉錄病毒載體的構建及表達[J];細胞與分子免疫學雜志;2012年07期
3 楊毅力,趙小俠;逆轉錄病毒載體的研究現(xiàn)狀及其在分子生物學中的應用[J];病毒學報;1991年03期
4 ;基因治療中逆轉錄病毒載體轉導及生產的改進方法[J];國外醫(yī)學(免疫學分冊);1994年04期
5 李楠,王鑫,馮東曉,李春海,徐采樸;組織金屬蛋白酶抑制劑逆轉錄病毒載體的構建[J];軍事醫(yī)學科學院院刊;1996年01期
6 傅建新,王瑋,盧大儒,岑建農,陳子興;增強型綠色熒光蛋白逆轉錄病毒載體的構建和表達[J];中國實驗血液學雜志;2000年04期
7 孫世波;邰升;張新宇;;人白細胞介素-10基因的克隆與逆轉錄病毒載體的構建[J];中華實驗外科雜志;2006年09期
8 孔令雄;吳e，
本文編號：2418277
本文鏈接：http://www.sikaile.net/xiyixuelunwen/2418277.html
最近更新
教材專著
