【摘要】：目的觀察三七對(duì)腦梗死模型小鼠的影響,探討作用機(jī)制。方法將50只SD小鼠隨機(jī)分成正常組、模型組、三七高劑量組、三七低劑量組和西藥組。通過(guò)線栓法構(gòu)建小鼠腦梗死模型。成模后,治療組開始三七溶液灌胃給藥12 w。N組和CT組給予等體積的生理鹽水溶液。給藥結(jié)束后觀察空腹血糖、肝、腎臟器系數(shù)、血清中白細(xì)胞介素(IL)-18、腫瘤壞死因子(TNF)-α以及腦組織中超氧化酶歧化酶(SOD)、過(guò)氧化氫酶(CAT)等相關(guān)指標(biāo)的含量,并通過(guò)實(shí)時(shí)定量聚合酶鏈反應(yīng)(real-time PCR)檢測(cè)Bcl-2、Bax mRNA水平。結(jié)果三七高劑量組及西藥組空腹血糖比模型組有顯著下降,而且三七治療組及西藥組均可升高Bcl-2的表達(dá)、下調(diào)Bax mRNA的表達(dá)(P0.05,P0.01)。結(jié)論三七可能通過(guò)升高Bcl-2的表達(dá)、下調(diào)Bax mRNA的表達(dá),從而減輕局灶性缺血性小鼠腦梗死。
[Abstract]:Objective to observe the effect of Panax notoginseng on cerebral infarction mice and explore the mechanism. Methods 50 SD mice were randomly divided into normal group, model group, high dose group, low dose group and western medicine group. The model of cerebral infarction in mice was established by the method of thread embolization. After the model was established, the treatment group was given the same volume of normal saline solution by intragastric administration of Panax notoginseng solution 12 w.N and CT group. After administration, fasting blood glucose, liver and kidney organ coefficients, serum interleukin (IL) 18, tumor necrosis factor (TNF) 偽 and superoxidase dismutase (SOD), in brain tissue were observed. The content of catalase (CAT) and Bcl-2,Bax mRNA were detected by real-time quantitative polymerase chain reaction (real-time PCR). Results compared with the model group, the fasting blood glucose in the high dose group and the western medicine group of Panax notoginseng decreased significantly, and the expression of Bcl-2 was increased and the expression of Bax mRNA was down-regulated in the treatment group and the western medicine group (P0.05, P0.01). Conclusion Panax notoginseng may reduce focal ischemic cerebral infarction by increasing the expression of Bcl-2 and down-regulating the expression of Bax mRNA.
【作者單位】： 河南省中醫(yī)院腦病科;
【基金】：河南省中醫(yī)藥科學(xué)研究專項(xiàng)課題(2015zy2023)
【分類號(hào)】：R285.5;R-332

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