發(fā)布時間：2018-10-24 07:01

【摘要】：目的 觀察靶向干擾前列腺癌抗原-1(PCA-1)表達對前列腺癌裸鼠移植瘤的影響。 方法 培養(yǎng)人前列腺癌LNCaP細胞并將其注射到裸鼠皮下建立前列腺癌裸鼠移植瘤模型。將裸鼠隨機分為實驗組和對照組,實驗組：將靶向干擾PCA-1表達的重組質(zhì)粒PSMAe/p-PCA-1-pA-Sliencer注射到裸鼠移植瘤局部和周圍,對照組：將PBS注射到移植瘤局部和周圍；觀察移植瘤生長情況和實驗結(jié)束時腫瘤的大小。應(yīng)用免疫組織化學(xué)方法和常規(guī)病理技術(shù)觀察腫瘤組織中PCA-1表達情況以及裸鼠主要器官的病理變化。 結(jié)果 實驗組裸鼠移植瘤生長速度明顯慢于對照組,差異具有統(tǒng)計學(xué)意義(P0.05)；實驗結(jié)束時實驗組和對照組的移植瘤體積(cm3)分別為1.142±0.157和3.086±0.265,差異有統(tǒng)計學(xué)意義(P0.05)。實驗組裸鼠的主要器官未出現(xiàn)明顯病理變化。實驗組裸鼠移植瘤組織PCA-1蛋白染色平均總積分(3.8±1.48)高于對照組(6.2±2.48),差異具有統(tǒng)計學(xué)意義(P0.05)。 結(jié)論 成功建立前列腺癌裸鼠移植瘤模型,靶向干擾PCA-1表達可以抑制移植瘤的生長,對裸鼠主要器官未產(chǎn)生明顯影響。
[Abstract]:Objective to investigate the effect of target interfering prostate cancer antigen 1 (PCA-1) expression on transplanted prostate cancer in nude mice. Methods Human prostate cancer LNCaP cells were cultured and injected subcutaneously into nude mice to establish transplanted tumor model of prostate cancer in nude mice. The nude mice were randomly divided into experimental group and control group. In the experimental group, the recombinant plasmid PSMAe/p-PCA-1-pA-Sliencer, which targeted to interfere with the expression of PCA-1, was injected into and around the transplanted tumor of nude mice. In the control group, PBS was injected into and around the transplanted tumor. The growth of transplanted tumor and the size of tumor at the end of the experiment were observed. The expression of PCA-1 in tumor tissues and the pathological changes of the main organs of nude mice were observed by immunohistochemical method and routine pathological technique. Results the tumor growth rate of nude mice in the experimental group was significantly slower than that in the control group (P0.05), the cm3 of the experimental group and the control group were 1.142 鹵0.157 and 3.086 鹵0.265 respectively at the end of the experiment (P0.05). There were no obvious pathological changes in the main organs of the nude mice in the experimental group. The mean total score of PCA-1 protein staining in nude mice transplanted in the experimental group (3.8 鹵1.48) was significantly higher than that in the control group (6.2 鹵2.48) (P0.05). Conclusion the transplanted tumor model of prostate cancer was successfully established in nude mice. The targeted interference of PCA-1 expression could inhibit the growth of transplanted tumor, but had no obvious effect on the main organs of nude mice.
【學(xué)位授予單位】：鄭州大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2012
【分類號】：R737.25;R-332

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