【摘要】：目的:考察補腎活血化痰方對多囊卵巢綜合征(PCOS)模型大鼠的治療作用機制。方法:將50只大鼠隨機分為正常對照組、模型對照組、陽性對照組[羅格列酮,3.0 mg/(kg·d)]和補腎活血化痰方高、低劑量組[7.5、5.0 g/(kg·d)],每組10只。除正常對照組外,其余各組大鼠均采用Poresky法復(fù)制PCOS模型。造模后,正常對照組和模型對照組大鼠ig生理鹽水,各給藥組大鼠ig相應(yīng)藥液,每天1次,連續(xù)22 d。給藥結(jié)束后,測定大鼠血清中睪酮(T)、抗苗勒氏管激素(AMH)、胰島素樣生長因子Ⅰ(IGF-Ⅰ)、腫瘤壞死因子α(TNF-α)水平和卵巢組織中轉(zhuǎn)化生長因子β1(TGF-β_1)陽性表達情況。結(jié)果:與正常對照組比較,模型對照組大鼠血清中T、AMH、IGF-Ⅰ、TNF-α水平及卵巢組織中TGF-β_1陽性表達率均顯著升高(P0.05);與模型對照組比較,各給藥組大鼠血清中T、AMH、IGF-Ⅰ、TNF-α水平及卵巢組織中TGF-β_1陽性表達率均顯著降低(P0.05),且補腎活血化痰方各劑量組指標(biāo)優(yōu)于陽性對照組(P0.05)。結(jié)論:補腎活血化痰方能明顯降低PCOS模型大鼠血清中T、AMH、IGF-Ⅰ、TNF-α水平以及卵巢組織中TGF-β_1陽性表達率,這可能是其治療PCOS的作用機制之一。
[Abstract]:Aim: to investigate the therapeutic mechanism of Bushen Huoxue Huatan recipe on (PCOS) model rats with polycystic ovary syndrome (PCOS). Methods: 50 rats were randomly divided into normal control group, model control group, positive control group [rosiglitazone 3.0 mg/ (kg d)] and high prescription for tonifying kidney and activating blood circulation to remove phlegm, low dose group [7.5 渭 g / (kg d)], 10 rats in each group. In addition to the normal control group, the other groups of rats using Poresky method to replicate the PCOS model. After the establishment of the model, normal control group and model control group rats ig normal saline, each group rats ig corresponding solution, once a day, continuous 22 days. After administration, the serum levels of testosterone (T), (AMH), insulin-like growth factor 鈪，

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