FGL2在泡球蚴感染所致DC細胞成熟中的作用研究
發(fā)布時間：2018-09-09 20:39
【摘要】：目的研究FGL2在泡球蚴感染所致DC細胞成熟中的作用及意義。方法 8~10周齡雌性野生型BALB/c小鼠12只和FGL2基因敲除小鼠12只,均隨機分為2組:感染組6只,對照組6只。實驗組腹腔注射0.1ml泡球蚴混懸液,對照組于相同部位注射等量PBS。感染后4個月將小鼠處死,取脾,制備脾細胞懸液,采用流式細胞術檢測DC細胞成熟表面標記CD80及CD86的表達。留取血清,采用ELISA測定FG12水平。結果泡球蚴載量感染晚期fgl2-/-小鼠低于野生型小鼠,(F=8.426,P0.05)。qRT-PCR檢測泡球蚴感染晚期Em14-3-3mRNA表達,fgl2-/-小鼠低于野生型小鼠,(F=16.070,P0.05);ELISA檢測泡球蚴感染早期野生型小鼠和對照組血清FGL2,感染組高于對照組(F=13.995,P0.05);流式細胞術檢測泡球蚴感染小鼠腹腔細胞和脾臟細胞中CD11b+DC和CD11c+DC細胞CD80表達水平發(fā)現(xiàn)前者泡球蚴感染fgl2-/-小鼠晚期腹腔細胞中CD80表達水平顯著高于野生型小鼠(F=236.303,P0.05),后者在泡球蚴感染fgl2-/-小鼠晚期腹腔細胞和脾臟細胞CD80的表達水平均顯著高于野生型小鼠(F=35.096,P0.05);ELISA檢測泡球蚴感染鼠中血清TNF-α水平發(fā)現(xiàn)泡球蚴感染fgl2-/-小鼠表達高于野生型小鼠(F=13.612,P0.05)。結論在泡球蚴感染小鼠晚期,FGL2高表達可能參與泡球蚴感染引起的DC細胞成熟度降低及由此所致的免疫逃避有關。
[Abstract]:Objective to study the role and significance of FGL2 in DC cell maturation induced by alveolar hydatid infection. Methods 12 female wild-type BALB/c mice and 12 FGL2 knockout mice were randomly divided into two groups: infection group (n = 6) and control group (n = 6). The experimental group was intraperitoneally injected with 0.1ml alveolar hydatid suspension, while the control group was injected with the same amount of PBS. at the same site. Four months after infection, mice were killed and spleen cells were taken to prepare spleen cell suspensions. Flow cytometry was used to detect the expression of CD80 and CD86 on the mature surface of DC cells. The serum was collected and the level of FG12 was determined by ELISA. Results the load of alveolar hydatid in late stage fgl2-/- mice was lower than that in wild type mice. (FG 8.426 P 0.05). QRT-PCR was used to detect the expression of Em14-3-3mRNA in late stage of alveolar hydatid infection. The expression of Em14-3-3mRNA in mice with late infection was lower than that in wild type mice. (FF16. 070%) Elisa was high in early stage wild-type mice infected with alveolar echinococci and control group in serum FGL2, infection. The expression level of CD11b DC and CD11c DC cells in peritoneal cells and spleen cells of mice infected with alveolar echinococcosis was detected by flow cytometry. It was found that the expression of CD80 in the late stage of fgl2-/- mice infected with alveolar hydatid was significantly higher than that in mice infected with wild field hydatid. The expression of CD80 in the late stage of fgl2-/- mice infected with alveolar hydatid was significantly higher than that in wild-type mice (FF35.096P05). The expression of TNF- 偽 in serum of mice infected with alveolar echinococci was detected by Elisa. The expression of TNF- 偽 in mice infected with alveolar echinococci was found to be higher than that in mice infected with fgl2-/-. It was higher than that of wild type mice (FG 13.612 P 0.05). Conclusion the high expression of FGL2 in the late stage of alveolar hydatid infection may be involved in the decrease of DC cell maturity caused by alveolar hydatid infection and the immune evasion caused by it.
【作者單位】： 新疆醫(yī)科大學基礎醫(yī)學院組織學與胚胎學教研室;新疆醫(yī)科大學第一附屬醫(yī)院新疆包蟲病基礎醫(yī)學重點實驗室;
【基金】：新疆維吾爾自治區(qū)自然科學基金面上項目(No.2014211C035)
【分類號】：R383.3
本文編號：2233506
[Abstract]:Objective to study the role and significance of FGL2 in DC cell maturation induced by alveolar hydatid infection. Methods 12 female wild-type BALB/c mice and 12 FGL2 knockout mice were randomly divided into two groups: infection group (n = 6) and control group (n = 6). The experimental group was intraperitoneally injected with 0.1ml alveolar hydatid suspension, while the control group was injected with the same amount of PBS. at the same site. Four months after infection, mice were killed and spleen cells were taken to prepare spleen cell suspensions. Flow cytometry was used to detect the expression of CD80 and CD86 on the mature surface of DC cells. The serum was collected and the level of FG12 was determined by ELISA. Results the load of alveolar hydatid in late stage fgl2-/- mice was lower than that in wild type mice. (FG 8.426 P 0.05). QRT-PCR was used to detect the expression of Em14-3-3mRNA in late stage of alveolar hydatid infection. The expression of Em14-3-3mRNA in mice with late infection was lower than that in wild type mice. (FF16. 070%) Elisa was high in early stage wild-type mice infected with alveolar echinococci and control group in serum FGL2, infection. The expression level of CD11b DC and CD11c DC cells in peritoneal cells and spleen cells of mice infected with alveolar echinococcosis was detected by flow cytometry. It was found that the expression of CD80 in the late stage of fgl2-/- mice infected with alveolar hydatid was significantly higher than that in mice infected with wild field hydatid. The expression of CD80 in the late stage of fgl2-/- mice infected with alveolar hydatid was significantly higher than that in wild-type mice (FF35.096P05). The expression of TNF- 偽 in serum of mice infected with alveolar echinococci was detected by Elisa. The expression of TNF- 偽 in mice infected with alveolar echinococci was found to be higher than that in mice infected with fgl2-/-. It was higher than that of wild type mice (FG 13.612 P 0.05). Conclusion the high expression of FGL2 in the late stage of alveolar hydatid infection may be involved in the decrease of DC cell maturity caused by alveolar hydatid infection and the immune evasion caused by it.
【作者單位】： 新疆醫(yī)科大學基礎醫(yī)學院組織學與胚胎學教研室;新疆醫(yī)科大學第一附屬醫(yī)院新疆包蟲病基礎醫(yī)學重點實驗室;
【基金】：新疆維吾爾自治區(qū)自然科學基金面上項目(No.2014211C035)
【分類號】：R383.3
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