【摘要】：目的探討帕金森病(PD)模型小鼠脾懸液中輔助性T細(xì)胞17(Th17)和髓系抑制性細(xì)胞(MDSC)數(shù)量的變化及其意義。方法將40只C57BL/6小鼠隨機(jī)分為PD組(A組)、PD+吉西他濱組(B組)、吉西他濱組(C組)與對照組(D組),每組10只小鼠。A組和B組予1-甲基-4-苯基-1,2,3,6-四氫吡啶(MPTP)(30 mg/kg)連續(xù)腹腔注射7 d;B組和C組于第1 d,7 d腹腔注射吉西他濱(120 mg/kg);D組予等體積生理鹽水腹腔注射。第8 d進(jìn)行行為學(xué)實(shí)驗(yàn)后,檢測各組小鼠黑質(zhì)區(qū)酪氨酸羥化酶(TH)陽性細(xì)胞數(shù)量和脾懸液Th17和MDSC細(xì)胞百分率。結(jié)果與建模前比較,A組、B組小鼠調(diào)頭時(shí)間、爬桿時(shí)間明顯延長,懸掛時(shí)間明顯縮短(均P0.05)。與C組、D組比較,建模后A組、B組小鼠調(diào)頭時(shí)間、爬桿時(shí)間明顯延長,懸掛時(shí)間明顯縮短(均P0.05)。A組、B組小鼠中腦黑質(zhì)區(qū)TH陽性細(xì)胞數(shù)量較C組和D組明顯減少(均P0.05)。A組、B組小鼠脾懸液中Th17、MDSC細(xì)胞百分?jǐn)?shù)較C組和D組明顯升高(均P0.05);且B組小鼠脾懸液中Th17細(xì)胞百分?jǐn)?shù)明顯高于A組(P0.05),A組小鼠脾懸液中MDSC細(xì)胞百分?jǐn)?shù)明顯高于B組(P0.05)。Pearson相關(guān)性分析結(jié)果顯示,A組和B組小鼠Th17和MDSC細(xì)胞百分?jǐn)?shù)間均無相關(guān)性(r=-0.319,r=0.04,均P0.05)。結(jié)論 PD模型小鼠Th17、MDSC數(shù)量增加,可能參與了PD的發(fā)生發(fā)展。MDSC有可能發(fā)揮了抑制Th17擴(kuò)增的免疫調(diào)節(jié)作用。
[Abstract]:Objective to investigate the changes and significance of helper T cell 17 (Th17) and myeloid inhibitory cell (MDSC) (MDSC) in spleen suspension of (PD) mice with Parkinson's disease. Methods 40 C57BL/6 mice were randomly divided into PD group (group A), gemcitabine group (group B), gemcitabine group (group C) and control group (group D). Group B and group C received intraperitoneal injection of gemcitabine (120 mg/kg) intraperitoneally on the 7th day after 7 days of irradiation with normal saline of the same volume. The number of tyrosine hydroxylase (TH) positive cells in substantia nigra and the percentage of Th17 and MDSC cells in spleen suspension were measured after behavioral experiment on the 8th day. Results compared with those before modeling, the turning time, climbing time and suspending time of group A and B mice were significantly prolonged (P0.05). Compared with group C and group D, the time of turning head and climbing pole in group A and group B were significantly prolonged after modeling. The suspension time was significantly shortened (P0.05). The number of TH positive cells in the substantia nigra of group A was significantly lower than that in group C and group D (P0.05). The percentage of Th17,MDSC cells in splenic suspension of group A was significantly higher than that of group C and group D (P0.05). The percentage of Th17 cells in splenic suspension of group B was significantly higher than that in group A (P0.05). Pearson correlation analysis showed that there was no correlation between the percentage of Th17 and MDSC in group A and group B (r-0.319r0.04, P0.05). Conclusion the increased number of Th17,MDSC in PD model mice may be involved in the development of PD. MDSC may play an immunoregulatory role in inhibiting Th17 amplification.
【作者單位】： 江蘇大學(xué)附屬醫(yī)院神經(jīng)內(nèi)科;江蘇大學(xué)附屬醫(yī)院中心實(shí)驗(yàn)室;
【基金】：江蘇省自然科學(xué)基金(BK20160548) 江蘇省“333工程”科研項(xiàng)目(BRA2014173) 江蘇省“六大人才高峰”科研項(xiàng)目(WSN038) 鎮(zhèn)江市重點(diǎn)研發(fā)計(jì)劃(SH2016032) 鎮(zhèn)江市社會發(fā)展科技計(jì)劃項(xiàng)目(SH2015047)
【分類號】：R-332;R742.5

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