發(fā)布時間：2018-08-20 17:03

【摘要】：目的:觀察低氧條件對小鼠脂肪間充質干細胞(ADSCs)膜片生物學性能的影響。方法:分離培養(yǎng)C57/BL小鼠ADSCs并用Vit C法構建細胞膜片;氯化鈷(CoCl_2)模擬體內低氧環(huán)境,q PCR和Western Blot檢測HIF-1α表達;組織學染色、透射電鏡觀察膜片形成;q PCR檢測ADSCs干性及膜片分泌功能相關基因的表達。結果:成功構建ADSCs膜片;HIF-1α的表達隨CoCl_2濃度的升高而升高;低氧條件下,形成細胞膜片較薄,但膜片內細胞連接更緊密;低氧環(huán)境可增強ADSCs干性,促使ADSCs膜片合成更多黏附分子、分泌更多保護性生長因子。結論:低氧條件更利于ADSCs膜片構建、存活和生物學功能維持。
[Abstract]:AIM: To observe the effects of hypoxia on the biological properties of adipose-derived mesenchymal stem cells (ADSCs) membrane in mice. Methods: ADSCs from C57/BL mice were isolated and cultured, and the cell membrane was constructed by Vit C method. Results: ADSCs membrane was successfully constructed, the expression of HIF-1a increased with the increase of CoCl_2 concentration, the formation of cell membrane was thinner under hypoxic condition, but the membrane cells were more closely connected; hypoxic environment could enhance the dryness of ADSCs, and promote the synthesis of more adhesion molecules and secretion of ADSCs membrane. CONCLUSION: Hypoxia is more conducive to the construction, survival and biological function of ADSCs.
【作者單位】： 軍事口腔醫(yī)學國家重點實驗室 口腔疾病國家臨床醫(yī)學研究中心 陜西省口腔生物工程技術研究中心 第四軍醫(yī)大學口腔醫(yī)院種植科;第四軍醫(yī)大學唐都醫(yī)院血液科;
【基金】：國家自然科學基金(編號:81170984,81470775)
【分類號】：R329.2
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本文編號：2194352