【摘要】：目的:探究體外誘導造血干細胞向紅系分化過程中AB血清對終末期紅系分化進程的影響。方法:從臍帶血中用免疫磁珠法分離提取CD34+細胞,體外誘導其向紅系分化。根據(jù)培養(yǎng)體系中是否添加AB血清,設置實驗組為添加AB血清組,對照組為不添加AB血清組。通過檢測細胞表面紅系分化標志分子糖化血紅蛋白A(glycophorin A,GPA)、人紅細胞陰離子交換蛋白Band3及整合素α4(α4-integrin)的表達反映終末紅系分化進程,用流式細胞術檢測紅系細胞終末分化脫核情況。結果:與對照組相比,實驗組GPA陽性細胞百分率較高,Band3表達較高,而α4-integrin表達較低(P0.05)。實驗組細胞脫核百分率明顯高于對照組(P0.05)。結論:在體外紅系發(fā)育培養(yǎng)體系中,AB血清對終末期紅系分化進程有一定的促分化、促脫核作用。
[Abstract]:Aim: to investigate the effect of AB serum on the process of erythroid differentiation induced by hematopoietic stem cells in vitro. Methods: CD34 cells were isolated from umbilical cord blood by immunomagnetic beads and induced to differentiate into erythrocytes in vitro. According to whether the AB serum was added in the culture system, the experimental group was added AB serum group, and the control group was no AB serum group. The expression of human erythrocyte anion exchange protein (Band3) and integrin 偽 4 (偽 4-integrin) were detected to reflect the process of terminal erythroid differentiation. Flow cytometry was used to detect the denucleation of erythrocyte terminal differentiation. Results: compared with the control group, the percentage of GPA positive cells in the experimental group was higher than that in the control group, while the expression of 偽 4-integrin was lower (P0.05). The percentage of cell denucleation in the experimental group was significantly higher than that in the control group (P0.05). Conclusion: in vitro erythroid development culture system, the serum Asab can promote the differentiation and denucleation of the end-stage erythroid.
【作者單位】： 中南大學生命科學學院分子生物學研究中心;中南大學湘雅醫(yī)學院;中南大學湘雅醫(yī)院血液科;
【基金】：國家自然科學基金(81270576) 中南大學校級大學生創(chuàng)新訓練計劃項目(YC13296)~~
【分類號】：R329.2
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本文編號：2176729