【摘要】：第一章HIF-1α、IL-8在肺低氧代謝中的表達(dá) 目的研究肺低氧代謝中血清中缺氧誘導(dǎo)因子1α(HIF-1α),促炎細(xì)胞因子白介素-8(IL-8)表達(dá),探討低氧代謝與其相關(guān)性。 方法40只SD大鼠隨機(jī)分為對(duì)照組(A組),實(shí)驗(yàn)組:30min單肺低氧組(B組),60min單肺低氧組(C組),120min單肺低氧組(D組);A組采用氣管切開(kāi)插管行雙肺通氣,實(shí)驗(yàn)組行氣管切開(kāi)插管行單肺通氣,建立AHPI模型,分別于實(shí)驗(yàn)30min、60min、120min取大鼠血液及非通氣側(cè)肺組織,通過(guò)透射電鏡檢查非通氣側(cè)肺組織細(xì)胞結(jié)構(gòu)變化,采用ELISA法,Western blot法測(cè)定HIF-1α,IL-8含量。 結(jié)果A組鏡下可見(jiàn)AEC-Ⅱ的核質(zhì)均勻,可見(jiàn)特有的L(blamellar body板層小體)。B組AEC-Ⅱ微絨毛(Microvilli Mv)排列混亂,Lb排空略微增強(qiáng);C組Lb數(shù)目增加,排空增強(qiáng);D組核固縮,出現(xiàn)巨大的Lb,伴空泡樣變。實(shí)驗(yàn)組肺組織及血清中HIF-1α的蛋白含量明顯高于對(duì)照組且隨低氧代謝時(shí)間延長(zhǎng)呈上升趨勢(shì):ABCD (P0.01),而IL-8的含量與對(duì)照組相比卻無(wú)明顯差異(P0.05)。 結(jié)論低氧代謝致大鼠肺組織細(xì)胞結(jié)構(gòu)發(fā)生改變,HIF-1α的表達(dá)上調(diào),且與低氧代謝時(shí)間呈正相關(guān)性。 第二章TGF-β1、AIF在肺低氧代謝中的表達(dá) 目的研究肺低氧代謝中肺泡上皮Ⅱ型細(xì)胞(alveolar epithelial cells type II,AEC-Ⅱ)的超微結(jié)構(gòu)變化與肺組織及血清中轉(zhuǎn)化生長(zhǎng)因子β1(Transforming growth factor-β1,TGF-β1)和凋亡誘導(dǎo)因子(apoptosis inducing factor ,AIF)表達(dá),探討其之間關(guān)系。 方法40只SD大鼠隨機(jī)均分為A,B,C,D組(n=10),A、B、C組采用氣管切開(kāi)插管行單肺通氣,建立AHPI模型,D組采用氣管切開(kāi)插管行雙肺通氣。分別于實(shí)驗(yàn)30min(A)、60min(B)、120min(C,D)取大鼠血液,離心取血清凍存,取左下肺組織待透射電鏡檢查和蛋白含量測(cè)定。用ELISA法,Western blot法分別測(cè)定在血清和肺組織的TGF-β1、AIF表達(dá)。 結(jié)果A組AEC-Ⅱ微絨毛(Microvilli Mv)排列混亂,Lb數(shù)目增多,排空略微增強(qiáng)。B組Lb數(shù)目增加,密度明顯降低,排空增強(qiáng),部分呈空泡樣變。C組核固縮,未見(jiàn)核仁,出現(xiàn)巨大的Lb,伴空泡樣變。D組鏡下可見(jiàn)AEC-Ⅱ的細(xì)胞核,核質(zhì)均勻,胞質(zhì)內(nèi)可見(jiàn)AEC-Ⅱ特有的Lb(lamellar body板層小體),無(wú)空泡樣變及排空現(xiàn)象。A、B、C組肺組織與血清中TGF-β1、AIF的相對(duì)含量明顯高于D組且隨缺氧時(shí)間延長(zhǎng)呈上升趨勢(shì):DABC (P0.01)。 結(jié)論低氧代謝致大鼠肺組織細(xì)胞結(jié)構(gòu)發(fā)生改變,TGF-β1和AIF的含量在試驗(yàn)時(shí)間內(nèi)表達(dá)呈上調(diào)趨勢(shì)。
[Abstract]:Chapter 1 expression of HIF-1 偽 and IL-8 in hypoxic Metabolism objective to study the expression of hypoxia inducible factor-1 偽 (HIF-1 偽) and pro-inflammatory cytokine interleukin-8 (IL-8) in serum of pulmonary hypoxia metabolism, and to explore the correlation between hypoxia metabolism and hypoxia metabolism. Methods Forty Sprague-Dawley rats were randomly divided into two groups: control group (group A), control group (group A, n = 30 min), group B (group B, n = 60), group A (n = 120 min), group A (n = 120), group A were treated with tracheotomy and intubation, and group A, were treated by tracheotomy and intubation, respectively. The AHPI model was established. The blood and non-ventilated lung tissues of the rats were collected at 30min or 60min for 120min, respectively. The changes of cellular structure in the non-ventilated lung tissues were examined by transmission electron microscopy. The content of HIF-1 偽 -IL-8 was measured by ELISA method. Results in group A, the nucleus and cytoplasm of AEC- 鈪，

本文編號(hào)：2142508