本文選題：急性痛風性關節(jié)炎 + 大鼠模型��； 參考：《中國實驗動物學報》2017年05期

【摘要】：目的建立急性痛風性關節(jié)炎(acute gouty arthritis,AGA)大鼠模型并觀察其維持時間。方法采用25 mg/m L尿酸鈉(monosodium urate,MSU)晶體混懸液踝關節(jié)腔注射復制大鼠AGA模型,多個時間點動態(tài)觀察8 d,以大鼠受試踝關節(jié)局部皮溫、腫脹度、步態(tài)、關節(jié)液炎性細胞及其滑膜組織病理形態(tài)學改變等指標判斷是否成模及其維持時間。結果造模后3 h,生理鹽水組和模型組均可見踝關節(jié)腫脹,皮溫升高,步態(tài)異常,關節(jié)液炎性細胞數(shù)增多,滑膜組織增生、毛細血管充血、滑膜細胞排列紊亂等炎癥表現(xiàn),兩組以上指標與空白組比較差異均有顯著性(P0.01);造模后4 h,生理鹽水組以上炎癥表現(xiàn)明顯減輕,較3 h時差異有顯著性(P0.01),而模型組較3h時加重(P0.01),并且與生理鹽水組比較差異有顯著性(P0.01);造模后24 h,生理鹽水組各項指標恢復正常,而模型組炎癥繼續(xù)加重;造模后48~72 h,模型組腫脹、皮溫、步態(tài)異常等局部炎癥達到高峰;造模后96~168h,模型組踝關節(jié)局部炎癥逐漸減輕,但各項指標與空白組比較差異仍有顯著性(P0.01);造模后192 h,模型組腫脹、皮溫、步態(tài)異常等外在炎癥表現(xiàn)恢復正常,而炎性細胞數(shù)及滑膜病理變化與空白組比較差異仍均有顯著性(P0.01)。結論采用MSU晶體混懸液踝關節(jié)腔注射可在造模后4 h成功制備并鑒定出AGA大鼠模型,且至少能維持到造模后168 h。
[Abstract]:Objective to establish the model of acute gouty arthritis (AGA) rats with acute gouty arthritis and observe its maintenance time. Methods the rat model was made by injection of 25 mg/m L sodium uric acid sodium monosodium urateate (MSU) crystal suspension into the ankle joint. Dynamic observation was made at multiple time points for 8 days. The local skin temperature, swelling degree and gait of the ankle joint were observed at different time points. The pathological and morphological changes of synovium and inflammatory cells in articular fluid were used to determine whether the model was formed and the time of maintenance. Results the swelling of ankle joint, elevation of skin temperature, abnormal gait, increased number of inflammatory cells in synovial fluid, proliferation of synovial tissue, capillary hyperemia, disorder of synovial cell arrangement and other inflammatory manifestations were observed in saline group and model group 3 hours after modeling. The above indexes in the two groups were significantly different from those in the blank group (P 0.01), and the inflammatory manifestations of the above groups in the saline group were significantly reduced at 4 h after the establishment of the model. There was a significant difference in P0.01D between the model group and the saline group at 3 h, and the difference was significant in the model group compared with the saline group at 3 h, the indexes of the saline group returned to normal at 24 h after modeling, but the inflammation in the model group continued to increase. The swelling, skin temperature and gait abnormality reached the peak in the model group at 48h 72 h after modeling, and the local inflammation of the ankle joint decreased gradually at 96h 168h after modeling, but there were significant differences between the indexes of the model group and the blank group (P 0.01), the swelling of the model group at 192 h after modeling. The number of inflammatory cells and the pathological changes of synovial membrane were significantly different from those of the blank group (P 0.01). Conclusion the rat model of AGA can be successfully prepared and identified by injection of MSU crystal suspension into ankle joint at 4 hours after the establishment of the model, and can be maintained at least 168 hours after the establishment of the model.
【作者單位】： 福建中醫(yī)藥大學;福建衛(wèi)生職業(yè)技術學院;
【基金】：國家自然科學基金資助項目(No.81473495)
【分類號】：R-332;R589.7
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本文編號：2017095