本文選題：αA腎上腺素能受體 + 星形膠質細胞�。� 參考：《醫(yī)學研究雜志》2016年09期

【摘要】：目的驗證大鼠大腦皮質星形膠質細胞中的α2A腎上腺素能受體(α2A-AR)的表達情況,并與α2B腎上腺素能受體(α2B-AR)、α2C腎上腺素能受體(α2C-AR)相比較。方法獲取目的基因α2A-AR、α2B-AR、α2C-AR的RNA反轉錄,與內參基因β-actin一起進行實時熒光定量PCR。重復6組,所得數(shù)據(jù)進行統(tǒng)計學分析并運用2-△△Ct的方法進行計算。結果大鼠大腦皮質星形膠質細胞中確實表達了α2A-AR,并且α2A-AR的相對表達量是α2B-AR的24.53倍,是α2C-AR的27.98倍。結論α2A-AR可能為大鼠大腦皮質星形膠質細胞接受兒茶酚胺類神經遞質調控的主要受體。
[Abstract]:Objective to investigate the expression of 偽 2A adrenergic receptor (偽 2A-ARR) in rat cerebral cortex astrocytes and compare it with 偽 2B adrenoceptor (偽 2B-ARA, 偽 2C adrenoceptor (偽 2C-ARR). Methods RNA reverse transcriptions of target genes 偽 2A-AR, 偽 2B-ARand 偽 2C-AR were obtained, and real-time quantitative PCRs were performed with 尾 -actin. Repeat 6 groups, the data were statistically analyzed and calculated by 2-Ct method. Results 偽 2A-AR was expressed in astrocytes of rat cerebral cortex, and the relative expression of 偽 2A-AR was 24.53 times of 偽 2B-AR and 27.98 times of 偽 2C-AR. Conclusion 偽 2A-AR may be the main receptor of catecholamine neurotransmitters in astrocytes of rat cerebral cortex.
【作者單位】： 遵義醫(yī)學院、貴州省麻醉與器官保護基礎研究重點實驗室;
【基金】：國家自然科學基金資助項目(81460219)
【分類號】：R338
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本文編號：2000291