本文選題：NKT細(xì)胞 + 凋亡��； 參考：《中國(guó)輸血雜志》2016年01期

【摘要】：目的針對(duì)NKT細(xì)胞培養(yǎng)擴(kuò)增和培養(yǎng)過(guò)程中遇到的過(guò)早凋亡現(xiàn)象,在凋亡信號(hào)層面進(jìn)行初步的描述和探討。為今后的NKT相關(guān)研發(fā)提供數(shù)據(jù)參考。方法自健康人濃縮白細(xì)胞制品(白膜)分離有外周血單個(gè)核細(xì)胞(peripheral blood mononuclear cells PBMNCs),利用其中的抗原遞呈細(xì)胞,以α-Gal Cer的刺激NKT細(xì)胞克隆產(chǎn)生,記錄3-4周NKT的表型變化,和Caspase-3酶活化及細(xì)胞凋亡狀況。結(jié)果刺激7 d后,NKT克隆開(kāi)始產(chǎn)生,持續(xù)擴(kuò)增3周左右后開(kāi)始呈現(xiàn)凋亡狀態(tài),Caspase3酶在第2周即開(kāi)始維持高活性狀態(tài)。人NKT細(xì)胞對(duì)α-Gal Cer的刺激的反應(yīng)具有很大的個(gè)體差異。結(jié)論 NKT細(xì)胞克隆可以再最初的3周內(nèi)如同常規(guī)T細(xì)胞克隆一樣生長(zhǎng),其后則會(huì)因α-GalCer刺激引發(fā)活化后凋亡。
[Abstract]:Objective to investigate the phenomenon of premature apoptosis in the process of NKT cell proliferation and culture. For the future NKT related research and development to provide data reference. Methods Peripheral blood mononuclear cells (PBMNCs) were isolated from the white membrane of healthy people. The antigen-presenting cells were used to produce NKT cells stimulated by 偽 -Gal Cer, and the phenotypic changes of NKT were recorded at 3-4 weeks. And Caspase-3 enzyme activation and apoptosis. Results after stimulation for 7 days, NKT clones began to produce, and then continued to amplify for about 3 weeks. The apoptotic state of Caspase3 enzyme began to maintain high activity at the second week. The response of human NKT cells to 偽 -Gal Cer is very different. Conclusion NKT cell clone can grow as conventional T cell clone in the first 3 weeks, and then apoptosis after activation induced by 偽 -GalCer stimulation.
【作者單位】： 上海市血液中心;
【基金】：上海市自然科學(xué)基金面上項(xiàng)目(132R1438900) 上海市衛(wèi)生局青年科研項(xiàng)目資助,No.024Y37 上海市血液中心科技發(fā)展基金項(xiàng)目資助,No.L(9)
【分類(lèi)號(hào)】：R392.12

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