本文選題：肝纖維化 + 肝星狀細(xì)胞��； 參考：《中南大學(xué)》2012年碩士論文

【摘要】：目的：觀察AKF-PD對大鼠肝星狀細(xì)胞增殖的影響,初步探討其機(jī)制 方法： 1.原位灌流法從S-D大鼠肝臟內(nèi)分離原代大鼠肝星狀細(xì)胞。 2.分離培養(yǎng)14天的原代肝星狀細(xì)胞分為：正常組、模型組(10ng/ml PDGF、200μg/ml AKF-PD治療組(10ng/ml PDGF+200μ g/ml AKF-PD)、400μg/ml AKF-PD治療組(10ng/ml PDGF+400μg/ml AKF-PD),藥物預(yù)處理24小時后加入PDGF10ng/ml繼續(xù)培養(yǎng)24小時,MTT法檢測AKF-PD對大鼠肝星狀細(xì)胞增殖的影響。 3.將分離培養(yǎng)14天的原代肝星狀細(xì)胞分為正常組、模型組(10ng/ml PDGF)、AKF-PD治療組(10ng/ml PDGF+400μg/ml AKF-PD)、 PFD治療組(10ng/ml PDGF+400μg/ml PFD)。藥物預(yù)處理24小時后加入PDGF10ng/ml繼續(xù)培養(yǎng)24小時,提取細(xì)胞蛋白,Wesern Blot法檢測CyclinD1、CyclinE、P27Kip1蛋白表達(dá)。 結(jié)果： 1.與正常組比較,模型組肝星狀細(xì)胞吸光度值顯著增加；與模型組比較,不同濃度的AKF-PD治療組肝星狀細(xì)胞吸光度值均明顯下降,且400μg/ml AKF-PD治療組較200μg/ml AKF-PD治療組下降明顯。 2.與正常組比較,模型組肝星狀細(xì)胞CyclinD1、CyclinE表達(dá)明顯增加,P27Kip1表達(dá)明顯降低；與模型組比較,AKF-PD治療組CyclinD1、CyclinE表達(dá)顯著降低, P27Kip1表達(dá)顯著升高。 結(jié)論： 1. AKF-PD顯著抑制大鼠肝星狀細(xì)胞增殖。 2. AKF-PD可能是通過下調(diào)CyclinD1、CyclinE表達(dá)和上調(diào)P27Kip1表達(dá)抑制大鼠肝星狀細(xì)胞增殖。
[Abstract]:Objective: to observe the effect of AKF-PD on the proliferation of rat hepatic stellate cells and to explore its mechanism. Methods: 1. Primary rat hepatic stellate cells were isolated from S-D rat liver by in situ perfusion. 2. Primary hepatic stellate cells were isolated and cultured for 14 days. The model group was treated with 10ng / ml PDGF200 渭 g/ml AKF-PD. The 10ng / ml PDGF 200 渭 g/ml AKF-PDT 400 渭 g/ml AKF-PD treated group was treated with 10ng / ml PDGF 400 渭 g/ml AKF-PDT. The effect of AKF-PD on the proliferation of rat hepatic stellate cells was detected by MTT assay after 24 hours of pretreatment with PDGF10ng/ml. 3. The primary hepatic stellate cells cultured for 14 days were divided into normal group. The model group was treated with 10ng / ml PDGF400 渭 g/ml AKF-PDD, and the model group was treated with 10ng / ml PDGF 400 渭 g/ml PFD-PDD, and the PFD treated group was 10ng / ml PDGF 400 渭 g/ml PFDD. After pretreatment for 24 hours, PDGF10ng/ml was added to culture for 24 hours. The protein expression of Cyclin D _ (1) and Cyclin E _ (27) Kip1 was detected by Wesern Blot assay. Results: 1. Compared with the normal group, the absorbance of hepatic stellate cells in the model group was significantly increased, and the absorbance value of hepatic stellate cells in the different concentrations of AKF-PD treatment group was significantly lower than that in the 200 渭 g/ml AKF-PD group, and that in the 400 渭 g/ml AKF-PD treatment group was significantly lower than that in the 200 渭 g/ml AKF-PD treatment group. 2. Compared with the normal group, the expression of Cyclin D1 cyclin E in hepatic stellate cells in the model group increased significantly, and the expression of P27Kip1 decreased significantly in the model group, while the expression of Cyclin D1 and P27Kip1 increased significantly in the AKF-PD treatment group compared with the model group. Conclusion: 1. AKF-PD significantly inhibited the proliferation of rat hepatic stellate cells. 2. AKF-PD may inhibit the proliferation of rat hepatic stellate cells by down-regulating the expression of Cyclin E and up-regulating the expression of P27Kip1.
【學(xué)位授予單位】：中南大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2012
【分類號】：R363

【共引文獻(xiàn)】

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1 高俊茶;王妍;姜慧卿;;索拉非尼抑制人肝星狀細(xì)胞膠原合成[J];中國病理生理雜志;2012年01期

相關(guān)博士學(xué)位論文 前1條

1 高俊茶;多靶點(diǎn)激酶抑制劑sorafenib對肝纖維化大鼠及肝星狀細(xì)胞膠原代謝的影響及其機(jī)制的研究[D];河北醫(yī)科大學(xué);2010年

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本文編號：1920098