本文選題：動物模型 + 心肌無復(fù)流　； 參考：《中國動脈硬化雜志》2017年07期

【摘要】：目的制備大鼠心肌缺血再灌注損傷后無復(fù)流模型,探討建立模型過程中的關(guān)鍵點,為該疾病模型的成功復(fù)制提供參考。方法選取20只SD大鼠,隨機(jī)分成無復(fù)流組(n=15)和假手術(shù)組(n=5)。無復(fù)流組結(jié)扎冠狀動脈左前降支45 min后再灌注120 min;假手術(shù)組只穿線不結(jié)扎。在再灌注末予硫磺素S活體染色確定無復(fù)流區(qū),同時監(jiān)測大鼠呼吸及心電圖變化。結(jié)果 20只大鼠全部存活,且心電圖在心肌缺血期均出現(xiàn)心率加快、Ⅱ?qū)?lián)ST段抬高;恢復(fù)心肌灌注后,ST段下降30%;硫磺素S染色顯示出無復(fù)流區(qū)域。結(jié)論該方法可成功復(fù)制無復(fù)流動物模型,且模型制作方法成功率高,動物存活率也高。
[Abstract]:Objective to establish no reflow model after myocardial ischemia-reperfusion injury in rats, and to explore the key points in the process of establishing the model, and to provide a reference for the successful replication of the model. Methods Twenty Sprague-Dawley rats were randomly divided into no reflow group (n = 15) and sham operation group (n = 5). The left anterior descending coronary artery was ligated in no reflow group for 45 min and then reperfused for 120 minutes, while in the sham operation group, only the perforating line was not ligated. The no reflow area was determined by S staining at the end of reperfusion and the changes of respiration and electrocardiogram were monitored. Results all of the 20 rats survived, and the heart rate was increased in ECG during myocardial ischemia, St segment elevation in lead 鈪，

本文編號：1902960