本文選題：自由基 + 電子自旋共振　； 參考：《華東理工大學(xué)》2011年碩士論文

【摘要】：隨著自由基醫(yī)學(xué)和自由基生物學(xué)的發(fā)展,人們逐漸認(rèn)識到自由基與人體的多種生理病理現(xiàn)象之間有著密切的關(guān)系。從自由基角度,探索自由基損傷的預(yù)防、診斷和治療具有非常重要的意義。本論文一方面從檢測自由基損傷的角度,利用電子自旋共振(ESR)技術(shù)考察了N,N-二甲基甲酰胺(DMF)誘導(dǎo)小鼠急性應(yīng)激損傷模型中抗壞血酸及抗壞血酸自由基的變化,并以此作為自由基生物標(biāo)記物,探索出一種檢測DMF誘導(dǎo)氧化應(yīng)激自由基損傷的方法,對DMF誘發(fā)職業(yè)病的診斷及病理研究具有一定的意義。另一方面從預(yù)防和治療自由基損傷的角度,對外源性抗氧化劑進行了篩選�？疾炝�11種常見中草藥提取物對1,1-二苯基-2-苦基肼自由基、超氧陰離子自由基和羥基自由基的清除能力,并運用紫外可見分光光度法、化學(xué)發(fā)光法以及電子自旋共振檢測方法,綜合評價其體外抗氧化活性。結(jié)果顯示絕大多數(shù)中草藥均具有較好的抗氧化活性。其中,丁香、老鶴草、大黃清除自由基的能力最強。同時檢測結(jié)果表明,同種中草藥對不同自由基的清除能力存在一定差異,對同一自由基運用不同的檢測方法,結(jié)果的一致性也有所差異�；瘜W(xué)發(fā)光法與電子自旋共振技術(shù)相比,在檢測抗氧化劑清除活性氧自由基時更具有一致性。
[Abstract]:With the development of free radical medicine and free radical biology, people gradually realize that there is a close relationship between free radical and various physiological and pathological phenomena of human body. From the angle of free radical, it is very important to explore the prevention, diagnosis and treatment of free radical injury. In this paper, on the one hand, the changes of ascorbic acid and ascorbic acid free radicals in acute stress injury model of mice induced by N-dimethylformamide (DMF) were investigated by electron spin resonance (ESR) technique from the angle of free radical damage detection. As a free radical biomarker, a method of detecting oxidative stress free radical damage induced by DMF was explored, which is of significance for the diagnosis and pathological study of occupational diseases induced by DMF. On the other hand, exogenous antioxidants were screened from the angle of preventing and treating free radical injury. The scavenging ability of 11 kinds of Chinese herbal medicine extracts on 1-diphenyl-2-picrylhydrazine radical, superoxide anion radical and hydroxyl radical was investigated. The methods of UV-Vis spectrophotometry, chemiluminescence and electron spin resonance were used. Comprehensive evaluation of its antioxidant activity in vitro. The results showed that most Chinese herbs had good antioxidant activity. Among them, clove, old crane grass, rhubarb scavenging free radical ability is strongest. At the same time, the results showed that there were some differences in the scavenging ability of the same Chinese herbal medicine to different free radicals, and the consistency of the results was also different when different detection methods were used for the same free radicals. Compared with electron spin resonance (ESR), chemiluminescence method is more consistent in the detection of antioxidant scavenging of reactive oxygen radicals (Ros).
【學(xué)位授予單位】：華東理工大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2011
【分類號】：R363

【引證文獻】

相關(guān)期刊論文 前1條

1 田玉平;顧玲玲;;高效液相色譜法測定果汁飲料中的抗氧化劑[J];實驗室研究與探索;2012年10期

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