本文選題：滋腎育胎丸 + 免疫性不孕��； 參考：《中國(guó)中西醫(yī)結(jié)合雜志》2017年03期

【摘要】：目的探討滋腎育胎丸對(duì)抗精子抗體(As Ab)陽(yáng)性的不孕大鼠卵母細(xì)胞的干預(yù)作用。方法采用主動(dòng)免疫建立As Ab陽(yáng)性的免疫性不孕模型。將100只雌性SD大鼠隨機(jī)分為空白對(duì)照組、模型組及滋腎育胎丸低、中、高劑量組(簡(jiǎn)稱中藥低、中、高劑量組),每組20只。造模22天開(kāi)始灌胃給藥,模型組給予5 m L/kg生理鹽水灌胃,中藥低、中、高劑量組分別給予0.075、0.100、0.150 g/m L滋腎育胎丸灌胃,每天1次,至36天。分別在造模21天及36天采用ELISA法測(cè)定As Ab和轉(zhuǎn)化生長(zhǎng)因子(transforming growth factor-β,TGF-β)水平。處死大鼠后取卵母細(xì)胞,采用實(shí)時(shí)定量聚合酶鏈反應(yīng)(Real-time PCR)及Western blot分別檢測(cè)TGF-βmRNA及其蛋白表達(dá)水平;采用流式細(xì)胞學(xué)(FCM)和末端終未標(biāo)記(TUNEL)法測(cè)定卵母細(xì)胞凋亡率;采用細(xì)胞計(jì)數(shù)-8法(CCK-8)及5-乙炔基-2'脫氧尿嘧啶核苷(5-ethynyl-2'-deoxyuridine,Ed U)檢測(cè)卵母細(xì)胞增殖水平;采用劃痕實(shí)驗(yàn)及侵襲小室(Transwell)測(cè)定卵母細(xì)胞遷移與侵襲能力。結(jié)果與空白對(duì)照組比較,模型組卵母細(xì)胞凋亡增加,細(xì)胞增殖減少,遷移與侵襲能力減弱,TGF-βmRNA及其蛋白表達(dá)水平顯著下調(diào)(P0.05);與模型組比較,中藥高劑量組卵母細(xì)胞凋亡減少,細(xì)胞增殖增加,遷移與侵襲能力增強(qiáng),TGF-βmRNA及其蛋白表達(dá)水平顯著上調(diào)(均P0.05);且中藥高劑量組作用明顯優(yōu)于中藥低、中劑量組(P0.05)。結(jié)論應(yīng)用高劑量滋腎育胎丸干預(yù)免疫性不孕大鼠可明顯上調(diào)其卵母細(xì)胞TGF-β水平,改善卵母細(xì)胞功能。
[Abstract]:Objective to investigate the intervention effect of Zishen Yuet Wan (ZYT) on oocytes of infertile rats with positive sperm antibody (as Ab). Methods the immune infertility model with positive Asab was established by active immunity. 100 female Sprague-Dawley rats were randomly divided into blank control group, model group, low, medium and high dose groups (20 rats in each group). The model group was given intragastric administration of 5 m L/kg normal saline for 22 days, and the low, middle and high dose groups were given 0.075 ~ 0.100 ~ 0.150 g / ml Zishen Yu-fetus pill, once a day to 36 days. The levels of Asab and transforming growth factor- 尾 (TGF- 尾) were measured by ELISA method on the 21st and 36th day respectively. The expression of TGF- 尾 mRNA and its protein were detected by real-time quantitative polymerase chain reaction (PCR) and Western blot, and the apoptosis rate of oocytes was determined by flow cytology (FCM) and terminal unlabeled Tunel (Tunel). Cell count (CCK-8) and 5-ethynyl-2'deoxyuridine (Ed) were used to detect the proliferation of oocytes, and the migration and invasiveness of oocytes were measured by scratch test and invasive chamber transwelling. Results compared with the blank control group, the apoptosis of oocytes in the model group increased, the cell proliferation decreased, and the expression of TGF- 尾 mRNA and its protein decreased significantly in the model group, and the apoptosis of the oocytes in the high dose group decreased compared with that in the model group, and the expression of TGF- 尾 mRNA and its protein decreased in the model group. The expression level of TGF- 尾 mRNA and its protein was increased significantly (all P0.05%), and the effect of high dose group was better than that of low dose group, and the middle dose group was significantly higher than that of middle dose group (P0.05%). Conclusion the high dose Zishen Yuet pill can obviously upregulate the TGF- 尾 level of oocytes and improve the oocyte function in immunological infertility rats.
【作者單位】： 南方醫(yī)科大學(xué)附屬花都醫(yī)院圍產(chǎn)醫(yī)學(xué)中心;
【基金】：廣東省科技廳科技項(xiàng)目(No.2015A030302004,No.2015A030302003) 廣東省中醫(yī)藥局科技項(xiàng)目(No.20161191,No.20151061) 廣東省自然科學(xué)基金資助項(xiàng)目(No.2016A030313419)
【分類號(hào)】：R285.5;R-332

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