本文選題：脊髓損傷 + 慢性壓迫�。� 參考：《中國脊柱脊髓雜志》2017年03期

【摘要】：目的 :探討缺氧誘導因子-1α(hypoxia inducible factor-1α,HIF-1α)和基質(zhì)金屬蛋白酶-2(matrix metalloproteinases-2,MMP-2)在慢性壓迫性頸脊髓損傷大鼠模型中的表達及意義。方法 :將80只成年SD大鼠隨機分為假手術(shù)組和慢性壓迫性頸脊髓損傷組(脊髓壓迫組),每組40只。大鼠麻醉后充分顯露C5和C6椎板,切除C5左側(cè)半椎板,顯露硬脊膜,脊髓壓迫組在C6椎板和硬脊膜之間置入吸水后可膨脹聚氨酯薄板(1×3×1mm);假手術(shù)組只顯露硬脊膜不置入壓迫物。兩組分別通過BBB(Basso Beattie Bresnahan)評分和體感誘發(fā)電位(somatosensory evoked potential,SEP)檢測評估脊髓功能;于造模后7d、28d、42d和70d時處死大鼠取頸脊髓組織進行HIF-1α及MMP-2免疫組化染色,檢測其在各時間點的表達量(IOD值),采用獨立樣本t檢驗比較兩組各時間點的差異,分析HIF-1α、MMP-2與脊髓功能變化的相關(guān)性。結(jié)果:造模后7d時,兩組BBB評分無顯著性差異;SEP潛伏期顯著性延長,波幅顯著性降低;HIF-1α顯著性降低,MMP-2顯著性升高。28d時脊髓壓迫組BBB評分顯著性低于假手術(shù)組,SEP潛伏期與7d比較顯著性延長(P0.05)、波幅顯著性降低(P0.05),HIF-1α表達顯著性增高(P0.05),而MMP-2表達顯著性降低(P0.05)。42d時,脊髓壓迫組BBB評分、SEP潛伏期和波幅與28d時比較無顯著性差異,HIF-1α表達顯著性增高(P0.05),而MMP-2表達顯著性降低(P0.05);70d時脊髓壓迫組神經(jīng)功能較28d時顯著性改善,BBB評分顯著性增高(P0.05),SEP潛伏期顯著性縮短(P0.05)、波幅顯著性升高(P0.05);HIF-1α表達較前顯著性降低,而MMP-2表達升高,但與假手術(shù)組比較仍有顯著性差異(P0.05)。HIF-1α表達量與BBB評分呈顯著性負相關(guān)(r=-0.458,P0.05),MMP-2表達量與BBB評分呈顯著性正相關(guān)(r=0.903,P0.05)。結(jié)論:大鼠慢性壓迫性脊髓損傷后具有一定程度的自我修復能力,HIF-1α、MMP-2表達變化與慢性脊髓壓迫性損傷后神經(jīng)功能改善相關(guān)。
[Abstract]:Objective: to investigate the expression and significance of hypoxia inducible factor-1 偽 (HIF-1 偽) and matrix metalloproteinases-2 (MMP-2) in chronic compressive cervical spinal cord injury (CSCI) rats. Methods: 80 adult SD rats were randomly divided into sham operation group and chronic compression cervical spinal cord injury group (40 rats in each group). After anesthesia, C5 and C6 laminae were fully exposed, the left half lamina of C5 was excised and the dura dural was exposed. In the spinal cord compression group, 1 脳 3 脳 1mm-1 mm expandable polyurethane thin plate was placed between the C6 lamina and the dura mater, and the sham operation group only exposed the dura mater not placed into the compression material. The spinal cord function was evaluated by BBB(Basso Beattie Bresnahan score and somatosensory evoked potentialsep, and the cervical spinal cord tissues were sacrificed for HIF-1 偽 and MMP-2 immunohistochemical staining on the 7th day, 28d, 42d and 70d, respectively. The expression of HIF-1 偽 MMP-2 was measured at different time points, and the correlation between HIF-1 偽 MMP-2 and spinal cord function was analyzed by t test. Results: there was no significant difference in BBB scores between the two groups at 7 days after modeling. The BBB score of spinal cord compression group was significantly lower than that of sham operation group (P 0.05), and the amplitude was significantly lower than that of sham operation group (P 0.05), while the expression of HIF-1 偽 in spinal cord compression group was significantly increased (P 0.05) and MMP-2 was significantly higher than that of sham operation group (P < 0.05), and the amplitude was significantly lower than that of sham operation group (P < 0.05), while the expression of HIF-1 偽 in spinal cord compression group was significantly higher than that in sham operation group (P < 0.05). The expression of P0. 05 and P0. 05 was significantly decreased on day 42, and the expression of P0. 05 was significantly decreased. There was no significant difference in latency and amplitude of BBB between spinal cord compression group and 28d group. The expression of HIF-1 偽 was significantly increased in spinal cord compression group. However, the expression of MMP-2 was significantly decreased at 70 days after spinal cord compression. The neurological function of spinal cord compression group was significantly improved compared with that at 28 days. The latent period of SEP was significantly shortened and the expression of HIF-1 偽 was significantly decreased. The expression of MMP-2 was increased, but there was still significant difference between the expression of P0.05U. HIF-1 偽 and BBB score. There was a significant negative correlation between the expression of MMP-2 and the BBB score. There was a significant positive correlation between the expression of MMP-2 and the BBB score. Conclusion: the expression of HIF-1 偽 and MMP-2 is related to the improvement of neural function after chronic compressive spinal cord injury in rats.
【作者單位】： 中山大學附屬第一醫(yī)院脊柱外科;廣東省骨科學重點實驗室;
【分類號】：R651.2;R-332

【相似文獻】

相關(guān)期刊論文 前10條

1 陳同度,張昌穎;素食大鼠的貧血現(xiàn)象[J];營養(yǎng)學報;1957年04期

2 陳偉強;趙善廣;;自制注射用大鼠固定裝置[J];上海實驗動物科學;1992年04期

3 肖柳英,林培英,馮昭明,張丹;不同周齡的SD大鼠生理、生化及體重的正常值測定[J];中藥新藥與臨床藥理;1996年03期

4 李淑云;簡易大鼠灌胃器的制作[J];錦州醫(yī)學院學報;2001年04期

5 楊明智,陳積圣;一種大鼠抓取與固定的新工具介紹[J];上海實驗動物科學;2001年03期

6 戴英,陸群;復方H_(505)對Wistar大鼠外周血的血液流變學指標的影響[J];中國血液流變學雜志;2001年01期

7 韋應(yīng)波,孫喜慶,曹新生,姚永杰,馮岱雅,楊長斌;+Gz暴露時間對大鼠記憶功能和行為的影響[J];航天醫(yī)學與醫(yī)學工程;2003年01期

8 呂學軍,郭俊生,李敏,周利梅,張永娟;暈船大鼠體內(nèi)鐵含量的變化[J];中國職業(yè)醫(yī)學;2003年04期

9 湯仁仙,王迎偉,王慧,周峰;201A中藥合劑對大鼠抗腎小球基底膜腎炎病變的影響[J];徐州醫(yī)學院學報;2003年06期

10 孫同柱,付小兵,翁立新,梁雪梅,陳偉;介紹一種簡易的大鼠保定方法[J];上海實驗動物科學;2004年01期

相關(guān)會議論文 前10條

1 尹音;孫振宇;胡敏;李冬霞;;持續(xù)性高正加速度對大鼠顳頜關(guān)節(jié)損傷的作用[A];第八屆全國顳下頜關(guān)節(jié)病學及（牙合）學大會論文匯編[C];2011年

2 祝~=驤;iJ梊霞;洃克琴;崔素英;文允摪;，

本文編號：1794028