本文選題：白假絲酵母菌 + 莢膜　； 參考：《贛南醫(yī)學院學報》2016年06期

【摘要】：目的:研究從淋病患者陰道分泌物中分離鑒定的具有莢膜的白假絲酵母菌蛋白質(zhì)組學特征。方法:采用二維凝膠電泳(2-DE)技術(shù)分離從淋病患者陰道分泌物中分離鑒定的具有莢膜的白假絲酵母菌(C1-4)和無莢膜的白假絲酵母菌(ATCC14053)的菌體蛋白質(zhì),用Image Master 2D platinum 7.0軟件識別差異蛋白點,MALDI-TOFMS鑒定差異蛋白。結(jié)果:C1-4與ATCC14053相比,顯著差異表達蛋白點232個,其中180種蛋白表達明顯上調(diào),52種蛋白表達明顯下調(diào),質(zhì)譜鑒定了32差異蛋白質(zhì)點,8個得到鑒定,均在C1-4菌株顯著表達。結(jié)論:具有莢膜的白假絲酵母菌與無莢膜的白假絲酵母菌菌株相比,蛋白質(zhì)表達存在明顯差異。
[Abstract]:Aim: to study the proteomic characteristics of Candida albicans isolated from vaginal secretions of gonorrhea patients.Methods: the proteins of Candida albicans C1-4 with capsule and ATCC14053 without capsule were isolated from vaginal secretions of gonorrhea patients by two-dimensional gel electrophoresis (2-DEE) technique.Differential proteins were identified by Image Master 2D platinum 7.0 software and MALDI-TOFMS.Results compared with ATCC14053, there were 232 differentially expressed protein spots in 1: C1-4, of which 180 proteins were up-regulated and 52 proteins were down-regulated. Mass spectrometry identified 32 differentially expressed protein spots, 8 of which were identified, all of which were significantly expressed in C1-4 strain.Conclusion: the protein expression of Candida albicans with capsule was significantly different from that of C. albicans without capsule.
【作者單位】： 贛南醫(yī)學院第一附屬醫(yī)院;贛南醫(yī)學院;
【基金】：江西省自然科學基金項目(20114BAB215023) 江西省教育廳科技計劃項目(GJJ10573、GJJ12550)
【分類號】：R379

【相似文獻】

相關(guān)期刊論文 前10條

1 高碩;趙瑋;邱榮敏;林艷;;白假絲酵母菌分泌型天冬氨酸蛋白酶與重癥嬰幼兒齲的相關(guān)性[J];中華口腔醫(yī)學研究雜志(電子版);2010年05期

2 周笑梅;寧玉梅;;單味中藥抑制白假絲酵母菌作用的研究進展[J];中華中醫(yī)藥學刊;2011年03期

3 張琳;孫s，

本文編號：1753316