本文選題：共振光散射光譜法　切入點(diǎn)：厚樸酚　出處：《湘潭大學(xué)》2011年碩士論文

【摘要】：共振光散射技術(shù)的近些年在各分析領(lǐng)域應(yīng)用較廣泛,以方法靈敏度高、操作簡(jiǎn)便、儀器簡(jiǎn)單、分析速度快等許多優(yōu)點(diǎn)引起科學(xué)研究者的注意。本文主要以抗炎和抗病毒藥為研究對(duì)象,采用共振光散射技術(shù)并結(jié)合紫外吸收分光光度法和量子化學(xué)計(jì)算方法,研究了它們的光譜性質(zhì),影響因素及相關(guān)機(jī)理,從而建立了測(cè)定的新方法。 本文主要有五部分組成,具體內(nèi)容如下: 第一章綜述了共振光散射光譜(RLS)法的發(fā)展、原理、定量分析基礎(chǔ);以及厚樸酚與和厚樸酚、α-倒捻子素、柚皮素、山奈酚等分析的研究進(jìn)展。 第二章研究了厚樸酚及和厚樸酚與陽(yáng)離子染料結(jié)晶紫(CV)和陰離子表面活性劑十二烷基硫酸鈉(SDS)形成的三元離子締合物的RLS特性,結(jié)合紫外吸收分光光度法對(duì)三者之間的作用機(jī)理進(jìn)行了初步探討,并對(duì)一些影響因素進(jìn)行了優(yōu)化。在pH=3.5的HAc-NaAc緩沖溶液介質(zhì)中,結(jié)晶紫對(duì)十二烷基硫酸鈉與厚樸酚/和厚樸酚的共振光散射體系有協(xié)同增強(qiáng)作用。在333 nm共振波長(zhǎng)處產(chǎn)生的RLS強(qiáng)度最強(qiáng),在0.02～5.33μg/mL范圍內(nèi)呈較好的線性關(guān)系,檢出限1.88×10~(-4)μg/mL,回收率為98.1%～103.0%,RSD為2.32% 第三章研究了茜素紅(AR)-十六烷基三甲基溴化銨(CTMAB)-α-倒捻子素三元體系的紫外吸收光譜和RLS特性,以及影響因素和最佳實(shí)驗(yàn)條件。在pH=7.6的Na_2CO_3-NaHCO_3緩沖介質(zhì)中,在331 nm△IRLS (體系的共振光強(qiáng)度變化)與α-倒捻子素在0.082～5.68μg/mL范圍內(nèi)呈線性關(guān)系,檢出限為2.83 ng/mL,回收率97.8%～101.4%,RSD為1.7%。 第四章研究了鋁(Ⅲ)-柚皮素-十二烷基苯磺酸鈉(SDBS)三元體系的RLS特性,影響因素及最佳反應(yīng)條件。實(shí)驗(yàn)結(jié)果表明,在pH=3.5的BR緩沖溶液中,在323 nm處△IRLS與柚皮素在0.027～6.80μg/mL范圍內(nèi)呈線性關(guān)系,檢出限為1.9 ng/mL,回收率98.8%～101.0%,RSD為1.2%。 第五章研究了山奈酚的RLS特性,山奈酚在酸性條件下有很強(qiáng)的RLS值,而在堿性介質(zhì)中共振光消失。在pH=5.5的BR緩沖介質(zhì)中,并用量子化學(xué)計(jì)算方法對(duì)山奈酚分子內(nèi)和分子間氫鍵進(jìn)行了計(jì)算,初步探討了山奈酚共振光散射光譜形成的機(jī)理。
[Abstract]:Resonance Light scattering (RLS) technology has been widely used in various analytical fields in recent years, with high sensitivity, simple operation and simple instrument. Many advantages, such as high speed of analysis, have attracted the attention of scientific researchers. In this paper, antiinflammatory and antiviral drugs are mainly studied, and resonance light scattering (RLS) technique, combined with ultraviolet absorption spectrophotometry and quantum chemical calculation, is used. Their spectral properties, influencing factors and related mechanisms were studied, and a new method for their determination was established. There are five parts in this paper, the specific contents are as follows:. In the first chapter, the development, principle and basis of quantitative analysis of resonance light scattering spectroscopy (RLSs) method are reviewed, as well as the research progress in the analysis of honokiol and honokiol, 偽 -inverted twist, naringin, kaempferol, etc. In chapter 2, the RLS properties of ternary ion-association complexes formed by honokiol and honokiol with cationic dye crystal violet and anionic surfactant sodium dodecyl sulfate were studied. Combined with ultraviolet absorption spectrophotometry, the mechanism of action among the three was preliminarily discussed, and some influencing factors were optimized. In the HAc-NaAc buffer solution of pH=3.5, Crystal violet has synergistic effect on the resonance light scattering system of sodium dodecyl sulfate and honokiol / honokiol. The intensity of RLS produced at 333nm is the strongest, and the linear relationship is good in the range of 0.02 渭 g/mL. The detection limit was 1.88 脳 10 ~ (-4) 渭 g 路mL ~ (-1) and the recovery was 98.1% and the RSD was 2.32%. In chapter 3, the UV absorption spectra and RLS characteristics of the ternary system of alizarin red AR-cetyltrimethylammonium bromide (CTMAB- 偽 -inverted twist), the influencing factors and the optimum experimental conditions are studied. In the Na_2CO_3-NaHCO_3 buffer medium of pH=7.6, There was a linear relationship between 偽 -inverted twist at 0.082 渭 g/mL and resonance light intensity change at 331nm. The detection limit was 2.83 ng / mL, and the recovery rate was 97.8ng / mL. The recovery was 1.7ng-1 路mL ~ (-1). In chapter 4, the RLS properties, influencing factors and optimum reaction conditions of Al (鈪，

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