本文關鍵詞： 條件恐懼刺激 杏仁核 BrdU NeuN 免疫組化　出處：《中南大學》2011年碩士論文　論文類型：學位論文

【摘要】：目的：檢測條件恐懼刺激后大鼠杏仁核區(qū)神經干細胞的數量及神經干細胞分化為神經元的量的變化,探討條件恐懼刺激后大鼠杏仁核區(qū)神經干細胞的增殖及分化與恐懼記憶的關系。 方法：1.應用足底電擊配對單調聲刺激的方法建立大鼠條件恐懼記憶模型,并以呆立時間(freezing time)評價大鼠對條件恐懼刺激事件的記憶。2.應用免疫組化方法檢測條件恐懼刺激后不同時間點(1、7、14、21、30、60天)大鼠杏仁核區(qū)BrdU(外源性神經干細胞標記物)陽性細胞的表達量。3.應用免疫熒光雙標記法檢測條件恐懼刺激后不同時間點大鼠杏仁核區(qū)BrdU與NeuN(神經元標記物)雙標陽性細胞的量。4.應用SPSS15.0統(tǒng)計軟件進行統(tǒng)計學分析。 結果：1.行為學檢測結果顯示：1、7、14、21、30、60天時間點的實驗組大鼠在被重新放入電擊箱給予單調聲刺激后,八分鐘內的呆立時間明顯長于相應時間點的對照組,差異具有顯著性(P0.01)Do。2. BrdU的免疫組織化學結果顯示：在大鼠杏仁核區(qū)可見典型的BrdU陽性細胞,計數杏仁核區(qū)BrdU陽性細胞,發(fā)現條件恐懼刺激后各時間點實驗組大鼠杏仁核區(qū)BrdU免疫陽性細胞數少于相應對照組,其中1、7、14、21、30天差異具有顯著性(P0.05),60天實驗組與對照組的差異沒有統(tǒng)計學意義。3.BrdU與NeuN的免疫熒光雙標結果顯示：在大鼠杏仁核區(qū)可見明顯的BrdU與NeuN雙標陽性細胞,計數杏仁核區(qū)BrdU陽性細胞及BrdU與NeuN的雙標陽性細胞,發(fā)現條件恐懼刺激后各時間點實驗組大鼠杏仁核區(qū)BrdU和NeuN免疫熒光雙標陽性細胞數與BrdU陽性細胞總數的比值小于相應對照組,差異具有顯著性(P0.05)。 結論：條件恐懼刺激后大鼠呆立時間明顯延長,且杏仁核區(qū)細胞增殖減少。以上結果提示條件恐懼刺激有可能影響大鼠杏仁核區(qū)的神經元新生。
[Abstract]:Objective: to detect the number of neural stem cells (NSCs) in amygdala and the number of neural stem cells (NSCs) differentiating into neurons after conditioned fear stimulation. To investigate the relationship between fear memory and proliferation and differentiation of neural stem cells in amygdala region after conditioned fear stimulation. Methods 1. The conditioned fear memory model of rats was established by plantar shock matching monotone sound stimulation. The memory of conditioned fear stimulation events in rats was evaluated by standing time and freezing time. 2. Immunohistochemical method was used to detect BrdU (exogenous neural stem cell marker) positive in amygdaloid nucleus of rats at different time points after conditioned fear stimulation. The expression of sex cells was measured by immunofluorescence double labeling method. The amount of BrdU and Neun (neuron marker) double labeled positive cells in amygdaloid nucleus of rats at different time points after conditioned fear stimulation was detected. 4. SPSS15.0 statistical software was used for statistical analysis. Results: 1.Behavioral tests showed that the rats in the experimental group at 30 days after being put back into the electric shock box for monotone sound stimulation were significantly longer in eight minutes than those in the control group at the corresponding time point. The immunohistochemical results of BrdU showed that typical BrdU positive cells were found in the amygdala of rats, and the BrdU positive cells in the amygdala were counted. It was found that the number of BrdU immunoreactive cells in the amygdala of the experimental group was less than that of the corresponding control group at each time point after conditioned fear stimulation. There was no significant difference between the experimental group and the control group on the 30th day. 3. The immunofluorescence double labeling results of BrdU and NeuN showed that there were obvious double labeled BrdU and NeuN positive cells in the amygdala of rats. BrdU positive cells and double labeled positive cells of BrdU and NeuN in amygdala were counted. It was found that the ratio of the number of BrdU and NeuN double labeled positive cells to the total number of BrdU positive cells in the amygdaloid nucleus of the experimental group was lower than that of the corresponding control group at each time point after conditioned fear stimulation, and the difference was significant (P 0.05). Conclusion: the experimental results suggest that conditioned fear stimulation may affect the neuronal growth of amygdaloid area in rats.
【學位授予單位】：中南大學
【學位級別】：碩士
【學位授予年份】：2011
【分類號】：R363

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