本文關(guān)鍵詞： 頸脊髓損傷 半側(cè)挫傷 前肢運(yùn)動(dòng)功能 大鼠 組織學(xué)　出處：《中國脊柱脊髓雜志》2017年10期 　論文類型：期刊論文

【摘要】：目的 :建立大鼠頸脊髓半側(cè)挫傷模型,評(píng)價(jià)不同程度脊髓損傷大鼠的行為學(xué)和組織學(xué)改變。方法 :選取32只成年雄性Sprague-Dawley大鼠,體重為280~320g。按挫傷深度分為1.2mm組(n=12)和2mm組(n=12),并設(shè)立假手術(shù)組(n=8)對(duì)照。麻醉狀態(tài)下后路手術(shù)暴露大鼠C5左側(cè)脊髓,特制椎夾固定頸椎并連于大鼠立體定位架后置于脊髓打擊器平臺(tái)上,然后應(yīng)用直徑為1.5mm的圓柱形打擊頭在500mm/s的速度下分別以1.2mm和2mm打擊深度背側(cè)垂直挫傷大鼠C5脊髓左半側(cè),監(jiān)測(cè)最大打擊力和挫傷能量。每只大鼠損傷后進(jìn)行前肢運(yùn)動(dòng)功能評(píng)分,圓柱攀爬試驗(yàn)和梳理試驗(yàn)。傷后8周取材,在脊髓橫斷面組織學(xué)切片上測(cè)量損傷中心處脊髓損傷面積,以及灰、白質(zhì)殘存面積。結(jié)果:中度損傷組最大打擊力和挫傷能量分別為0.99±0.14N和(1.08±0.01)×10-5J,重度損傷組為1.53±0.22N和(8.56±0.02)×10-5J,兩組間打擊力和損傷能量具有統(tǒng)計(jì)學(xué)差異(P0.05)。術(shù)后對(duì)照組大鼠均未見明顯神經(jīng)功能障礙,前肢運(yùn)動(dòng)功能評(píng)分為16.29±0.49。損傷組大鼠均出現(xiàn)單側(cè)神經(jīng)功能障礙,損傷同側(cè)前肢運(yùn)動(dòng)功能評(píng)分中度損傷組為1.40±0.52,重度損傷組為0.83±0.25,兩組無統(tǒng)計(jì)學(xué)差異(P0.05)。傷后8周前肢運(yùn)動(dòng)功能評(píng)分中度損傷組為12.40±1.15,重度損傷組為8.41±0.83,對(duì)照組為17.00±0.00,梳理試驗(yàn)評(píng)分中度損傷組為3.80±0.60,重度損傷組為2.51±0.54,對(duì)照組為5.00±0.00,損傷同側(cè)前肢使用比例中度損傷組為(32.31±5.70)%,重度損傷組為(4.92±1.21)%,對(duì)照組為(49.42±1.20)%,各組間均有統(tǒng)計(jì)學(xué)差異(P0.05)。鏡下觀察損傷組大鼠脊髓組織都出現(xiàn)了明顯的半側(cè)空洞和結(jié)構(gòu)破壞,而對(duì)側(cè)脊髓結(jié)構(gòu)基本完整。在損傷中心處,中度損傷組脊髓背側(cè)束、背外側(cè)束和脊髓后角有破壞,重度損傷組脊髓僅殘留部分的腹外側(cè)束,對(duì)照組脊髓組織結(jié)構(gòu)完整。灰質(zhì)和白質(zhì)殘存面積在中度損傷組分別為1.45±0.11mm~2和4.13±0.29mm~2,重度損傷組分別為1.10±0.05mm~2和3.84±0.19mm~2,組間均存在統(tǒng)計(jì)學(xué)差異(P0.05)。結(jié)論:本研究成功建立了不同程度的大鼠頸脊髓半側(cè)挫傷模型,不同挫傷深度表現(xiàn)出不同程度的單側(cè)前肢運(yùn)動(dòng)功能障礙和脊髓組織破壞,為頸脊髓半側(cè)損傷模型提供研究基礎(chǔ)。
[Abstract]:Objective: to establish a rat model of cervical spinal cord contusion. Methods: 32 adult male Sprague-Dawley rats were selected. The body weight was 280 ~ 320g. According to the depth of contusion, it was divided into 1.2mm group (n = 12) and 2mm group (n = 12). Under anaesthesia, the left spinal cord of C5 rats was exposed, and the cervical spine was fixed by special clamps and attached to the stereotactic frame of the rat. Then the left side of the C5 spinal cord was injured with 1.5 mm diameter cylindrical head at 500 mm / s at 1.2 mm and 2 mm depth, respectively. The maximum impact force and contusion energy were monitored. The motor function scores of forelimbs, column climbing test and combing test were performed in each rat after injury. The materials were collected 8 weeks after injury. The area of spinal cord injury at the center of injury and gray were measured on the transverse section of spinal cord. Results: the maximum impact force and contusion energy of moderate injury group were 0.99 鹵0.14 N and 1.08 鹵0.01) 脳 10 ~ (-5) J, respectively. In severe injury group, 1.53 鹵0.22N and 8.56 鹵0.02) 脳 10 ~ (-5) J respectively. There was a statistical difference between the two groups in impact power and injury energy (P 0.05). No obvious neurological dysfunction was found in the control group after operation. The motor function score of forelimb was 16.29 鹵0.49.The unilateral neurological dysfunction was found in all rats in the injury group, and 1.40 鹵0.52 in the moderate injury group. There was no significant difference between the two groups in severe injury group (0.83 鹵0.25). The forelimb motor function score of moderate injury group was 12.40 鹵1.15 at 8 weeks after injury. The scores of severe injury group and control group were 8.41 鹵0.83and 17.00 鹵0.00respectively. The score of carding test was 3.80 鹵0.60 in moderate injury group and 2.51 鹵0.54 in severe injury group. The control group was 5.00 鹵0.00.The proportion of injury to ipsilateral forelimb was 32.31 鹵5.70 in moderate injury group and 4.92 鹵1.21% in severe injury group. The control group was 49.42 鹵1.20%, and there was statistical difference between the two groups (P 0.05). Under the microscope, the spinal cord tissue of the injured group showed obvious hemispheric cavity and structural damage. The contralateral spinal cord structure was basically intact. At the center of the injury, the dorsal spinal tract, the dorsal lateral tract and the posterior horn of the spinal cord were damaged in the moderate injury group, while in the severe injury group, only the ventrolateral tract of the remnant part of the spinal cord was found. The remaining areas of gray matter and white matter were 1.45 鹵0.11mmm2 and 4.13 鹵0.29mmm2 in moderate injury group, respectively. The severe injury group was 1.10 鹵0.05mm-2 and 3.84 鹵0.19mmm2, respectively. Conclusion: the model of cervical spinal cord contusion in different degree was established successfully in this study. Different contusion depth showed different degree of unilateral forelimb motor dysfunction and spinal cord tissue damage, which provided a basis for the study of cervical spinal cord injury model.
【作者單位】： 南方醫(yī)科大學(xué)南方醫(yī)院脊柱骨科;
【基金】：國家自然科學(xué)基金(81601904) 廣東省自然科學(xué)基金(2014A030313336)
【分類號(hào)】：R-332;R651.2
【正文快照】： 脊髓損傷(spinal cord injury,SCI)多由交通傷深度建立不同程度的大鼠C5脊髓半側(cè)挫傷,事故、墜落和運(yùn)動(dòng)損傷引起,因椎體骨折撞擊脊髓同時(shí)評(píng)價(jià)其損傷后的神經(jīng)功能和組織形態(tài)改變,導(dǎo)致脊髓挫傷。近40%的脊髓損傷發(fā)生于頸脊髓為不同程度頸脊髓半側(cè)挫傷動(dòng)物模型的應(yīng)用研究段[1],引

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