本文關(guān)鍵詞：NGF結(jié)合HA對糖尿病大鼠骨缺損愈合影響的研究　出處：《南華大學(xué)》2012年碩士論文　論文類型：學(xué)位論文

  更多相關(guān)文章： 神經(jīng)生長因子 羥基磷灰石 骨缺損 糖尿病

【摘要】：研究目的：建立糖尿病大鼠脛骨骨缺損模型，通過比較使用神經(jīng)生長因子（nerve growth factor，NGF）、羥基磷灰石（hyzdroxya patite，HA）以及兩者聯(lián)合運用后對骨缺損愈合過程中對大體形態(tài)學(xué)、影像學(xué)、堿性磷酸酶（alkaline osphatase，ALP）、骨痂中Ca含量、組織學(xué)等指標(biāo)的影響，探討聯(lián)合運用神經(jīng)生長因子及羥基磷灰石對糖尿病大鼠骨缺損的療效與機理。 材料和方法： 75只健康雄性8周齡SD大鼠，隨機分為鏈脲佐菌素(streptozotoein，STZ）注射組60只，正常對照組15只，STZ組一次性腹腔注射鏈脲佐菌素(streptozotoein，STZ）50mg/kg，正常組作為一次性腹腔注射等容量生理鹽水，STZ組注射STZ后3天后隨機選出血糖濃度高于16.8mmol/L的納入糖尿病組，血糖未達標(biāo)者再補充。將糖尿病組隨機分為B、C、D、E四組，每組15只。正常組隨機選15只血糖正常者為A組正常對照組。5組大鼠均建立右脛骨上段4mm骨缺損模型，按A、B、C、D、E字母順序分別于骨缺損局部使用，生理鹽水、生理鹽水、NGF、HA、NGF\HA。術(shù)后第4周、8周、12周各組分別隨機抽取5只大鼠靜脈采血.進行大體標(biāo)本、骨缺損部位X線、血清ALP檢測、骨痂Ca含量及組織學(xué)觀察。 結(jié)果：術(shù)后同一時間，大體觀察：糖尿病組B、C、D、E組局部骨缺損處新生骨組織體積、硬度逐漸增加，空白對照A組愈合優(yōu)于糖尿病對照B組，差于D、E組；X線片示：觀察B、C、D、E組骨缺損骨痂橫向與縱向模糊程度逐漸增加，空白對照A組愈合優(yōu)于糖尿病B、C組，差于D、E組。組織學(xué)觀察：B、C、D、E組骨小梁成熟度和密度逐漸增高，空白對照A組愈合優(yōu)于糖尿病B、C組，低于其余組。B、C、D、E組堿性ALP含量依次遞增。A、C組于第4周P0.05，無統(tǒng)計學(xué)意義。各組的血清ALP含量于第8周增加達到最高峰，其中E組最高，與其它四組比較血清中ALP含量差異具有統(tǒng)計學(xué)意義(P0.05)，，ALP于12W回落。各組大鼠新生骨痂Ca含量隨時間增加而增加，橫向?qū)Ρ雀鲿r期由小到大B、C、A、D、E。 結(jié)論： NGF與HA聯(lián)合運用具有良好的誘導(dǎo)成骨作用，使骨細胞增生活躍，加快糖尿病鼠骨缺損愈合。
[Abstract]:Objective: to establish a model of tibial bone defect in diabetic rats and to compare the use of nerve growth factor (NGF) with nerve growth factor (NGF). Hydroxyapatite hyzdroxya patitehae) and the combined use of both in the healing process of bone defects in the gross morphology, imaging. The effect of alkaline phosphatase (ALP) on the contents of Ca and histology in callus. To explore the therapeutic effect and mechanism of nerve growth factor and hydroxyapatite in treating bone defect in diabetic rats. Materials and methods: Seventy-five healthy male 8-week-old SD rats were randomly divided into streptozotoein STZ (STZ) injection group (n = 60) and normal control group (n = 15). Streptozotoein group (STZ group) was injected intraperitoneally with 50 mg / kg streptozotoein (STZ) and the normal group was treated as a single intraperitoneal injection of normal saline of equal volume. Three days after the injection of STZ, the STZ group was randomly selected as the diabetic group with blood glucose concentration higher than 16.8 mmol / L, and those who did not meet the standard of blood sugar were supplemented. The diabetic group was randomly divided into four groups. 15 rats in each group. 15 normal subjects were randomly selected as group A normal control group (group A). 4 mm bone defect model of right upper tibia was established in group A. The alphabetical sequence of E was used locally in bone defect, saline, normal saline, NGFHANGF\ HA. the 4th week after operation was 8 weeks. At 12 weeks, 5 rats were randomly selected for venous blood collection. The gross specimens, bone defect X-ray, serum ALP, callus Ca content and histology were observed. Results: at the same time after operation, gross observation: the volume and hardness of the new bone tissue in the local bone defect of the diabetic group were gradually increased, and the healing of the blank control group A was better than that of the diabetic control group B. The difference was worse than that in group E. X-ray examination showed that the degree of transverse and longitudinal ambiguity of bone defect callus was gradually increased in group A, and the healing of group A was better than that of group B C of diabetes mellitus, which was worse than that of group DX E. the histological observation showed that the bone defect in group A was better than that in group B (n = 6). The bone trabecular maturation and density increased gradually in group D, and the content of alkaline ALP in group A was higher than that in group A, which was higher than that in group B and C of diabetes mellitus, and the content of alkaline ALP in group D was higher than that in group B (P < 0.05). Group C at the 4th week P0.05, no statistical significance. The serum ALP content in each group reached the highest peak at the 8th week, among which group E was the highest. Compared with the other four groups, the serum ALP content was significantly lower than that of the other four groups (P 0.05). The Ca content of the callus increased with the increase of time. The horizontal comparison is made from small to large, from small to large, from small to large. Conclusion: the combination of NGF and HA can induce osteogenesis, activate the proliferation of bone cells and accelerate the healing of bone defect in diabetic rats.
【學(xué)位授予單位】：南華大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2012
【分類號】：R587.1;R-332

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