本文關(guān)鍵詞：安神定志靈對ADHD模型鼠前額葉、紋狀體各G蛋白亞基表達(dá)的影響　出處：《遼寧中醫(yī)雜志》2017年03期 　論文類型：期刊論文

  更多相關(guān)文章： 安神定志靈 SHR大鼠 注意缺陷多動障礙 G蛋白偶聯(lián)受體

【摘要】：目的:探討中藥復(fù)方安神定志靈對注意缺陷多動障礙動物模型SHR大鼠前額葉、紋狀體中Gαs、Gαi及Golf mRNA和蛋白表達(dá)的影響。方法:將SHR大鼠運用隨機(jī)區(qū)組分為模型組、利他林組、安神定志靈低劑量、中劑量、高劑量組,每組10只,另將10只WKY大鼠設(shè)為正常組。安神定志靈低、中、高劑量組分別按生藥量6.7、13.4、26.7 g·kg~(-1)灌胃,每日2次;利他林組以2 mg·kg~(-1)給予利他林灌胃,每日2次;模型組和正常對照組每日按體質(zhì)量給予等量雙蒸水灌胃,灌胃4周后處死。分別用RT-PCR和Western Blot檢測各組大鼠前額葉、紋狀體中Gαs、Golf及Gαi mRNA和蛋白的表達(dá)水平。結(jié)果:模型組大鼠除前額葉皮質(zhì)Gαi蛋白外,余G蛋白亞基mRNA和蛋白表達(dá)水平均明顯低于正常組(P0.05或P0.01)。經(jīng)治療后,利他林組和安神定志靈各劑量組有提高前額葉皮質(zhì)、紋狀體中三種G蛋白亞基Gαi mRNA和蛋白的表達(dá)水平趨勢或能顯著提高其表達(dá)水平(P0.05或P0.01)。結(jié)論:安神定志靈可以升高SHR大鼠前額葉皮質(zhì)、紋狀體Gαs、Golf及Gαi三者mRNA和蛋白的表達(dá)水平,提示安神定志靈可以激活突觸后膜多巴胺受體下游不同的G蛋白亞基,進(jìn)而影響下游的信號通路從而發(fā)揮治療作用。
[Abstract]:Objective: to study the effect of Anshendingziling on G 偽 s in prefrontal lobe and striatum of SHR rats with attention deficit hyperactivity disorder (ADHD). Methods: SHR rats were randomly divided into three groups: model group, Ritalin group, low dose group, middle dose group and high dose group. 10 rats in each group were divided into normal group (n = 10). The low, medium and high doses of Anshendingzhiling were administered intragastrically according to the raw dosage of 6.713.4 ~ 26.7 g 路kg ~ (-1) 路kg ~ (-1) 路kg ~ (-1) 路kg ~ (-1) 路L ~ (-1) 路L ~ (-1). Twice a day; Ritalin group (2 mg 路kg ~ (-1)) was given Ritalin twice a day. The model group and the normal control group were fed with the same amount of double-steamed water daily according to body mass. After 4 weeks of gastric perfusion, the rats were killed. The prefrontal lobes were detected by RT-PCR and Western Blot. Results: in the model group, the expression of G 偽 偽 I protein and G 偽 I protein in the prefrontal cortex of the rats was increased in addition to the G 偽 I protein in the prefrontal cortex. The level of mRNA and protein expression of residual G protein subunit was significantly lower than that of normal group (P0.05 or P0.01). After treatment, the prefrontal cortex was increased in Ritalin group and Anshendingzhiling group. The tendency of expression level of G 偽 I mRNA and protein in striatum may be significantly increased (P0.05 or P0.01). Conclusion: Anshendingzhiling can increase the prefrontal cortex of SHR rats. The expression levels of mRNA and protein in striatum G 偽 ssilon Golf and G 偽 I suggest that Anshendingzhiling can activate different G protein subunits downstream of dopamine receptor in postsynaptic membrane. Thus affecting the downstream signaling pathway to play a therapeutic role.
【作者單位】： 南京中醫(yī)藥大學(xué)中醫(yī)兒科研究所;
【基金】：國家自然科學(xué)基金(81273801,81503614) 高等學(xué)校博士學(xué)科點專項科研基金課題(20123237110002)
【分類號】：R285.5;R-332
【正文快照】： 注意缺陷多動障礙(attention deficit hyperactivitydisorder,ADHD)是兒童常見的神經(jīng)發(fā)育障礙性疾病,以多動、沖動及注意缺陷為主要表現(xiàn),并常伴發(fā)學(xué)習(xí)障礙、對立-違抗障礙、品行障礙等[1]。由于以鹽酸哌甲酯為代表的中樞神經(jīng)興奮藥作為治療ADHD的一線藥物,存在食欲抑制、睡眠

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