本文關(guān)鍵詞：外源性透明質(zhì)酸對(duì)兔骨髓間充質(zhì)干細(xì)胞定向分化為軟骨細(xì)胞的影響　出處：《青島大學(xué)》2011年碩士論文　論文類(lèi)型：學(xué)位論文

  更多相關(guān)文章： 骨髓間充質(zhì)干細(xì)胞 軟骨細(xì)胞 透明質(zhì)酸 分化

【摘要】：背景：骨髓間充質(zhì)干細(xì)胞有多向分化潛能,在不同的誘導(dǎo)條件下可以分化成許多不同的組織,近年來(lái)已成為組織工程軟骨構(gòu)建過(guò)程中最常用的種子細(xì)胞。 目的：通過(guò)分析外源性透明質(zhì)酸對(duì)兔骨髓間充質(zhì)干細(xì)胞體外增殖及定向分化為軟骨細(xì)胞的影響,探討關(guān)節(jié)腔內(nèi)環(huán)境對(duì)骨髓間充質(zhì)干細(xì)胞的作用。 方法：全骨髓法+貼壁培養(yǎng)法分離培養(yǎng)兔骨髓間充質(zhì)干細(xì)胞,取第4代細(xì)胞用于實(shí)驗(yàn),實(shí)驗(yàn)組細(xì)胞加入透明質(zhì)酸誘導(dǎo)液,以轉(zhuǎn)化生長(zhǎng)因子β3誘導(dǎo)組作為陽(yáng)性對(duì)照,陰性對(duì)照組加入常規(guī)培養(yǎng)液。分別于誘導(dǎo)后第7,14,21 d行甲苯胺藍(lán)染色檢測(cè)蛋白聚糖表達(dá),免疫組化染色及RT-PCR檢測(cè)細(xì)胞Ⅱ型膠原表達(dá)。 結(jié)果：經(jīng)透明質(zhì)酸誘導(dǎo)后,細(xì)胞增殖速度減慢,由長(zhǎng)梭形變?yōu)槎嘟切�、橢圓形,細(xì)胞外基質(zhì)呈甲苯胺藍(lán)異染性和Ⅱ型膠原免疫組化陽(yáng)性, RT-PCR檢測(cè)示Ⅱ型膠原mRNA表達(dá)陽(yáng)性,表現(xiàn)出軟骨細(xì)胞的分化特點(diǎn),但表達(dá)均比陽(yáng)性對(duì)照組弱。 結(jié)論：外源性透明質(zhì)酸具有誘導(dǎo)兔骨髓間充質(zhì)干細(xì)胞向軟骨細(xì)胞分化的能力,但比轉(zhuǎn)化生長(zhǎng)因子β3的誘導(dǎo)能力弱。結(jié)果提示,關(guān)節(jié)腔內(nèi)環(huán)境對(duì)骨髓間充質(zhì)干細(xì)胞向軟骨細(xì)胞分化有正性促進(jìn)作用,支持透明質(zhì)酸作為軟骨組織工程基質(zhì)使用。
[Abstract]:Background: bone marrow mesenchymal stem cells (BMSCs) have the potential to differentiate into many different tissues under different induction conditions and have become the most commonly used seed cells in the process of tissue engineering cartilage construction in recent years. Aim: to investigate the effects of exogenous hyaluronic acid on the proliferation and differentiation of rabbit bone marrow mesenchymal stem cells into chondrocytes in vitro. Methods: the rabbit bone marrow mesenchymal stem cells were isolated and cultured by whole bone marrow adherent culture method. The cells of the 4th passage were used in the experiment. The cells in the experimental group were added hyaluronic acid inducer. Transforming growth factor 尾 3 group was used as positive control group and negative control group was added into conventional culture medium. Toluidine blue staining was used to detect proteoglycan expression on day 71421 after induction. The expression of type 鈪，

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