本文關鍵詞：基于MALDI-TOF MS的弧菌識別能力分析　出處：《中國病原生物學雜志》2016年06期 　論文類型：期刊論文

  更多相關文章： 基質輔助激光解析飛行時間質譜 弧菌 識別能力 特異性

【摘要】：目的確定基質輔助激光解析飛行時間質譜(MALDI-TOF MS)-Biotyper系統(tǒng)對弧菌屬細菌的識別能力。方法采用Bruker Microflex LT系統(tǒng),通過構建30株弧菌的肽質量參考譜庫、完善Biotyper數(shù)據庫;采用77株弧菌對數(shù)據庫的識別能力進行評價;采用主譜圖預測聚類分析揭示其對弧菌屬、種水平的區(qū)分能力及肽質量譜與菌株毒力的相關性。結果 107株弧菌通過MALDI-TOF MS-Biotyper原數(shù)據庫系統(tǒng)鑒定,92.5%(99株)為屬水平識別,55.1%(59株)為種水平識別。用于外部驗證的77株菌搜索Biotyper原數(shù)據庫和補充完善后的數(shù)據庫,種水平識別率從53.2%提高至96.1%,提高42.9%;屬水平識別率從92.2%提高至100%,提高7.8%;聚類分析顯示弧菌屬中麥氏弧菌與溶藻弧菌、弗尼斯弧菌肽質量譜特異性差,是造成識別錯誤的主要因素;聚類信息顯示,肽質量譜與霍亂弧菌的產毒性不相關,與副溶血性弧菌毒力不相關。結論 MALDI-TOF MS-Biotyper系統(tǒng)可用于弧菌屬、種水平的快速識別。數(shù)據庫的完善程度是決定種水平識別能力的關鍵。
[Abstract]:Objective to determine the matrix assisted laser desorption time of flight mass spectrometry (MALDI-TOF MS). Methods Bruker Microflex LT system was used to identify Vibrio bacteria. The Biotyper database was improved by constructing the peptide reference spectrum library of 30 strains of Vibrio. 77 strains of Vibrio were used to evaluate the recognition ability of the database. The main spectrum analysis was used to predict the genus Vibrio. Results 107 strains of Vibrio vibrio were identified by MALDI-TOF MS-Biotyper primordial database system. 92.5 and 99 strains) belong to the genus level recognition (55.1 and 59). 77 strains of bacteria used for external verification were used to search the original Biotyper database and the supplementary and improved database. The recognition rate of species level increased from 53.2% to 96.1, and increased 42.9%. The recognition rate of genus level increased from 92.2% to 100, and increased by 7.8%. Cluster analysis showed that vibrio mycelia and vibrio alginolyticus had poor specificity in the quality spectrum of vibrio Furniss peptide, which was the main factor causing the recognition error. Cluster information showed that the peptide mass spectrum was not related to virulence of Vibrio cholerae, but not to virulence of Vibrio parahaemolyticus. Conclusion MALDI-TOF MS-Biotyper system can be used in Vibrio. The degree of perfection of database is the key to determine the ability of recognition of species level.
【作者單位】： 中國疾病預防控制中心傳染病預防控制所中國疾病預防控制中心傳染病預防控制國家重點實驗室和感染性疾病診治協(xié)同創(chuàng)新中心;遼寧出入境檢驗檢疫局;丹東出入境檢驗檢疫局;
【基金】：國家質檢總局科研計劃項目(No.2012IK170) 傳染病預防控制國家重點實驗室自主項目(No.2014SKLID102) 中國疾病預防控制中心傳染病預防控制所科研課題
【分類號】：R378.3
【正文快照】： *?**?弧菌(Vibrio)為菌體短小、彎曲成弧形或逗點狀,具有極端鞭毛、無芽孢,動力陽性,發(fā)酵葡萄糖的革蘭陰性菌�；【鷱V泛分布于自然界,是水產養(yǎng)殖中最為常見的致病菌,人類主要通過接觸或攝入污染的食物感染,引起急性腹瀉�；【�60余種,目前已確認有12種對人有致病性:霍亂弧

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