目的:制作多孔Se@SiO₂納米球涂層導(dǎo)尿管并探究其對(duì)尿道前列腺切除術(shù)(TURP)后前列腺部尿道部創(chuàng)面愈合速度的影響及分子機(jī)制,為減少前列腺術(shù)后并發(fā)癥提供新思路。方法:1、制備多孔Se@SiO₂納米球,通過XRD和TEM技術(shù)鑒定其微觀結(jié)構(gòu)。并利用DCFH-DA探針等技術(shù)檢測(cè)其活性氧(ROS)清除能力及生物安全性。再將多孔Se@SiO₂納米球溶于殼聚糖溶液中制作涂層導(dǎo)尿管。同時(shí)測(cè)定導(dǎo)尿管的涂層厚度、Se釋放速率及涂層穩(wěn)定性。2、構(gòu)建比格犬TURP手術(shù)模型,將其隨機(jī)分為Se@SiO₂組和對(duì)照組。通過HE染色等方法對(duì)比兩組創(chuàng)面修復(fù)速度及基底細(xì)胞增殖、遷移、分化程度的差異。通過ICP-AES測(cè)定組織、血液、尿液中Se殘余量,生化法測(cè)定比格犬的肝腎功能及抗氧化酶活性。3、通過免疫熒光染色等實(shí)驗(yàn)檢測(cè)創(chuàng)面組織內(nèi)巨噬細(xì)胞極化。通過流式細(xì)胞分析技術(shù)、Western blot等實(shí)驗(yàn)明確多孔Se@SiO₂納米球?qū)w外培養(yǎng)的巨噬細(xì)胞的極化方向和NF-κB通路的影響。結(jié)果:1、成功制作多孔Se@SiO<...

【文章頁數(shù)】：165 頁

【學(xué)位級(jí)別】：博士

【文章目錄】：
摘要
abstract
中文部分
    緒論
    第一部分: 多孔Se@SiO₂納米球和多孔Se@SiO₂納米球涂層導(dǎo)尿管的合成及鑒定
        引言
        材料和方法
            1. 實(shí)驗(yàn)材料與儀器
            2. 實(shí)驗(yàn)方法
        結(jié)果
            1. 多孔Se@SiO₂納米球涂層導(dǎo)尿管的制作
            2. 多孔Se@SiO₂納米球涂層導(dǎo)尿管的特性測(cè)定
            3. 高生物安全性的多孔Se@SiO₂納米球可抑制氧化應(yīng)激對(duì)M0型巨噬細(xì)胞的損傷
        討論
        結(jié)論
    第二部分: 多孔Se@SiO₂納米球涂層導(dǎo)尿管對(duì)比格犬前列腺切除術(shù)后的創(chuàng)面修復(fù)速度及巨噬細(xì)胞極化的影響
        引言
        材料和方法
            1. 實(shí)驗(yàn)材料與儀器
            2. 實(shí)驗(yàn)方法
        結(jié)果
            1. 留置不同導(dǎo)尿管對(duì)比格犬經(jīng)尿道前列腺切除術(shù)后創(chuàng)面修復(fù)速度的影響
            2. 多孔Se@SiO₂涂層導(dǎo)尿管對(duì)比格犬的生物相容性和抗氧化應(yīng)激能力的測(cè)定
            3. 多孔Se@SiO₂納米球涂層導(dǎo)尿管在比格犬創(chuàng)面巨噬細(xì)胞極化過程中的作用
        討論
        結(jié)論
    第三部分: 多孔Se@SiO₂納米球調(diào)控巨噬細(xì)胞極化方向的分子機(jī)制
        引言
        材料和方法
            1. 實(shí)驗(yàn)材料與儀器
            2. 實(shí)驗(yàn)方法
        結(jié)果
            1. 多孔Se@SiO₂納米球干預(yù)體外巨噬細(xì)胞在氧化應(yīng)激模型的極化的方向
            2. 多孔Se@SiO₂納米球?qū)奘杉?xì)胞內(nèi)ROS-F-κB信號(hào)通路的作用
        討論
        結(jié)論
    全文總結(jié)
英文部分
    Preface
    Part Ⅰ Synthesis and Identification of Porous Se@SiO₂ Nanospheres and CoatedCatheter
        Introduction
        Materials and Methods
            1. Experimental materials and instruments
            2. Experimental methods
        Results
            1. Production of porous Se@SiO₂ nanosphere-coated catheter
            2. Determination of characteristics of porous Se@SiO₂ nanosphere-coated catheter
            3. High-level biosafety porous Se@SiO₂ nanospheres could inhibit oxidative stressdamage to MO macrophages
        Discussion
        Conclusions
    Part Ⅱ Effect of Porous Se@SiO₂ Nanosphere-Coated Catheter on WoundHealing and Macrophage Polarization in Prostatic Urethra of Beagle DogsFollowing TURP
        Introduction
        Materials and Methods
            1. Experimental materials and instruments
            2. Experimental methods
        Results
            1. Effect of indwelling different catheters on the recovery speed of prostatic urethrawounds of beagle dogs following TURP
            2. Determination of biocompatibility and antioxidant ability of porous Se@SiO₂nanospheres-coated catheter
            3. The role of porous Se@SiO₂ nanosphere-coated catheter in the polarization ofmacrophages in the wound
        Discussion
        Conclusions
    Part Ⅲ Molecular Mechanism of Porous Se@SiO₂ Nanospheres RegulatingPolarization Direction of Macrophages
        Introduction
        Materials and Methods
            1. Experimental materials and instruments
            2. Experimental methods
        Results
            1. Porous Se@SiO₂ nanospheres regulated polarization of macrophages in oxidativestress in vitro
            2. Effect of porous Se@SiO₂ nanospheres on ROS-NF-κB signaling pathway inmacrophages
        Discussion
        Conclusions
        Full text summary
參考文獻(xiàn)
致謝
學(xué)術(shù)論文和科研成果目錄
八年制學(xué)位論文要求



本文編號(hào)：3808142